钙粘蛋白13与BCR／ABL融合基因在慢性粒细胞白血病患者中的表达及其相关性

Expression of the CDH13 gene and BCR/ABL fusion gene in chronic myeloid leukemia patients and their relationship

目的观察钙粘蛋白13（cadherin-13，CDH13）与BCR／ABL融合基因在不同临床阶段慢性粒细胞白血病（chronic myeloid leukemia，CML）患者中的表达变化及其相关性。方法利用EVA Green建立实时荧光逆转录聚合酶链反应（reverse transcription polymerase chain reaction，RT—PCR）检测CDH13、BCR／ABL和磷酸甘油醛脱氢酶（glyceraldehyde phosphate dehydrogenase，GAPDH）的方法，检测25例CMI，初发和25例CML急变患者的外周血样本中CDH13基因和BCR／ABL融合基因的表达，以及30名正常人外周血样本中CDH13基因的表达，以GAPDH为内参基因，各组基因表达水平的差异采用△△CT相对定量法。结果CMI，急变患者外周血BCR／ABL融合基因的表达水平是初发患者的4．72倍，CMI。初发和急变患者较正常人CDH13的表达水平降低，分别是正常人的36％和25％，CDH13的表达水平与BCR／ABL融合基因的表达负相关。结论CDH13在CML不同临床阶段都表达降低，这可能是CML粘附缺陷的原因之一，CDH13表达可能受到BCR／ABL融合基因的调控。

Objective To investigate the expression of the CDH13 gene and BCR/ABL fusion gene in chronic myeloid leukemia（CML） patients at different stages and explore their relationship. Methods RNA was isolated from peripheral blood in 30 healthy adults, 22 primary CML patients and 25 blastic crisis of CML patients. We examined the expression of the CDH13 gene and BCR/ABL fusion gene using EVA Green real-time reverse transcriptase-polymerase chain reaction （RT-PCR）. Results The expression of the BCR/ABL fusion gene in the patients with blastic crisis CML was 4. 72 folds as that of the patients with primary CML. The expression of the CDH13 gene in the primary and blastic crisis CML patients was significantly reduced to 36% and 25% of that in healthy controls, respectively. Moreover, negative correlation was found between the expressions of these two genes. Conclusion The study showed that the reduction of the CDH13 expression at different clinical stage of CML may account for the defective cell adhesion in CML, and the expression of the CDH13 gene was probably down-regulated by the BCR/ABL fusion gene.