白花丹对家兔骨折愈合的影响

Effects of Plumbago zeylanica Linn on fracture healing in rabbits

背景:白花丹的主要成分是白花丹醌,它的活性主要是抗白血病、抗癌(肝癌)、抗菌、抗生育、祛痰等,对心血管也有一定的作用。目的:观察白花丹对实验性家兔骨折愈合过程的影响。设计、时间及地点:随机对照动物实验,于2006-12/2007-02在广西桂林医学院药理学教研室完成。材料:日龄80～85d新西兰家兔8只,体质量2.1～2.5kg,雌雄各半。白花丹全草浸膏,1mL相当于生药5.5g,临用前以体积分数为0.5的乙醇按2∶1的比例配制,由广西桂林医学院药理学教研室制备。方法:取家兔8只,随机分为生理盐水对照组和白花丹水提取液给药组,每组4只。2组于右前肢小腿中段前内侧,尺桡骨结合点上方用骨钳横行截断桡骨,造成家兔实验性右前肢骨折模型。给药组家兔右前肢骨折处外用白花丹全草浸膏的体积分数为0.5的乙醇稀释液,对照组给予生理盐水,2次/d,连续15d。主要观察指标:分别测定给药前,给药后15,30d家兔血清碱性磷酸酶、血Ca2+、P3+及微量元素K+、Na+、Cl-、游离钙、总钙浓度,并观察右前肢骨折X射线影像学检查。结果:与对照组比较,骨X射线影像学检查显示给药组家兔骨折愈合较快,且血清学检查表明给药组家兔血清Ca2+浓度较对照组显著升高,给药前后比较差异有显著性意义(P<0.05)。结论:白花丹可以明显增加骨折家兔血钙浓度,对家兔骨折愈合有促进作用。

BACKGROUND： The essential component of Plumbago zeylanica Linn is plumbagin, which plays a role in anti-leukemia, antibiosis, antifertility, as well as cardiovascular. OBJECTIVE： To investigate the effects of Plumbago zey/anica Linn on the healing process of experimental rabbit fracture. DESIGN, TIME AND SETTING： Randomized control experiment of animal was performed at the Department of Pharmacology, Guilin Medical College between December 2006 and February 2007. MATERIALS： Eight New Zealand rabbits, 80 to 85-day-old, weighing 2.1-2.5 kg, half male and half female. METHODS： Eight rabbits were randomly divided into the control and experimental groups, with 4 animals in each group. Under sterile conditions, the experimental bone fracture model of rabbit was created by making a longitudinal incision in the medial of the middle portion of right foreleg, cutting off a point-foot radius with bone clamp, and suturing the incision. The rabbits in the experimental group were treated externally with the extraction of Plumbago zey/anica Linn in 50% of ethanol, twice a day for 15 days. The sodium chloride was administrated into rabbits in the control group. MAIN OUTCOME MEASURES： The serum alkaline phosphatase （S-ALP）, Ca2＋, p3＋ K＋, Na＋, Cl-, liberation and total calcium of the rabbit were measured at 15 and 30 days after drug administration, X-ray of the bones were performed at the fracture healing. RESULTS： Compared with the control group, the fracture healing of the experimental group was faster, and the serum examinations showed that the serum Ca2＋ levels of the experimental group was significantly increased by Plumbago zeylanica Linn there had significantly differences between prior to and after drug administration （P 〈 0.05）. , CONCLUSION： Plumbago zeylanica Linn can increase the Ca2＋ level, plays an accelerate role in the process of healing.