蛋白质组学在创伤后异位骨化调控蛋白研究中的意义

Significance of proteomics application in regulatory proteins of post-traumatic heterotopic ossification

异位骨化是指不应发生骨化的软组织内出现病理性骨组织形成,多见于骨骼肌肉创伤、骨关节术后、神经系统损伤等。文章总结了运用蛋白质组学技术分析、比较异位骨组织在不同形成阶段的调控蛋白质在种类、数量存在的差异,据此可以筛选出不同时间点差异表达的功能蛋白,并针对性地对其中感兴趣的蛋白进行鉴定和功能验证,这种方式有助于发现新的调控蛋白,了解各种蛋白质之间相互作用的关系。证实蛋白质组学在创伤后异位骨化调控蛋白的研究中有广阔的应用前景,这有助于进一步阐明创伤后异位骨形成机制以及探索新的生物分子调控位点。

Heterotopic ossification is the abnormal formation of true bone within extraskeletal soft tissues, which frequently showing a predilection instead for fascia, tendons, and other mesenchymal soft tissues. This paper summarized differential types and quantities of regulatory proteins expression at different stages of heterotopic ossification by proteomics techniques, and screened differential expression of functional proteins at different time points. Further more, identificated and validated certain proteins, which helpful to discovery new regulatory proteins and explore the mutual relationships among the proteins. Proteomics has extensive applications in regulatory proteins research of post-traumatic heterotopic ossification, can further elucidate the mechanisrn of post-traumatic ectopic bone formation and search for new regulatory biomolecule regulatory sites.