来源于门静脉癌栓的人肝癌细胞系CSQT-1的建立及生物学特性分析

Establishment and characterization of a human hepatocellular carcinoma cell line CSQT-1 derived from portal vein tumor thrombus

目的:从肝癌及门静脉癌栓中分别取材建立人肝癌细胞系,探讨其生物学特性。方法:取人肝癌及门静脉癌栓新鲜手术标本,利用胶原酶消化法进行肿瘤细胞原代培养并扩大克隆培养建系。采用光镜、电镜、染色体核型分析及异种移植瘤实验对新建细胞系生物学特性进行观察。结果:来源于门静脉癌栓的人肝癌细胞在体外稳定培养已经将近1年,传至100余代,命名为CSQT-1。该细胞系具有典型的恶性上皮细胞特征,其群体倍增时间为48h;染色体中位数为87～90,为亚四倍体;裸鼠皮下异种移植可形成移植瘤,该细胞系中CD133+表达比较稳定。结论:细胞系特征显示该细胞系是一株新建的来源于门静脉癌栓的人肝癌细胞系。

Objective：To establish a human hepatocellular carcinoma cell line derived from portal vein tumor thrombus and study its biological characteristics.Methods：Specimens of liver cancer and its metastasis-portal vein tumor thrombi（PVTT） were freshly resected during operation.The tissues were primary cultured by collagenase Ⅰ digestion method and proliferated.The cell line derived from PVTT grew well.The biological characteristics of the cells were studied by light microscopy,electron microscopy,chromosome analysis,and transplantation experiment.Results：The cell line derived from portal vein tumor thrombus,named CSQT-1,has been cultivated for one year and sub-cultured for more than 100 passages in vitro.The cells either grew as compact colonies（in clusters） or as a monolayered sheet,with a doubling time of 48 h,and exhibited a typical malignant epithelial morphology.The median range of chromosome number was 87-90.Tumor nodes were observed under the skin of nude mice after subcutaneous xenograft transplantation for a month.The established cell line stably expressed CD133.Conclusion：We have successfully established a human hepatocellular carcinoma cell line from PVTT.