肝细胞生长因子和乙酰肝素酶在喉鳞状细胞癌组织中的表达及其临床意义

The expression of hepatocyte growth factor and heparanase in laryngeal squamous cell carcinoma and its clinical significance

目的:研究喉鳞状细胞癌(LSCC)组织中肝细胞生长因子(hypatocyte cell growth factor HGF)和乙酰肝素酶(heparanase,Hpa)的表达及其临床意义,分析不同病理分级(高、中、低分化)LSCC组织中HGF和Hpa表达的差异,为临床治疗及防治喉癌转移、复发提供理论依据和新的思路。方法:采用免疫组织化学SP法检测40例接受手术治疗并经病理证实为原发性LSCC的病理组织石蜡标本,同期40例接受手术治疗并经病理证实为成人喉原发性乳头状瘤病理组织石蜡标本和12例癌旁正常喉黏膜组织石蜡标本中的HGF和Hpa表达,并结合临床资料进行分析。结果:HGF、Hpa在LSCC组中阳性表达率分别为77.5%、72.5%,与喉乳头状瘤组和癌旁正常喉黏膜组比较,均差异有统计学意义(均P<0.01)。且两者的表达与LSCC原发部位无明显的相关性(P>0.05),而与T分期呈正相关(P<0.05);有局部淋巴结转移组显著高于无局部淋巴结转移组,其比较差异有统计学意义(P<0.05);与细胞分化程度呈负相关(P<0.05)。在40例LSCC中,HGF和Hpa表达呈正相关关系(P<0.01)。结论:LSCC中存在HGF和Hpa的高表达,与良性组织差别显著,肿瘤组织中的表达水平与与淋巴结转移、病理分级、临床分期、T分级等有关,与肿瘤原发部位无关。HGF和Hpa在LSCC的表达呈正相关,可作为评估LSCC进展的有价值的指标。

Objective：To explore the discrepancy of HGF and Hpa expressed in laryngeal squamous cell carcinoma of different pathology grades （differentiation of high, moderate or poor）, which can provide the new thought and theory evidence for clinical treatment and control metastasis or palindromia. Method： Forty paraffin-embedded samples of laryngeal squamous cell carcinoma, forty paraffin-embedded samples of laryngeal papilloma and twelve paraffin-embedded samples of normal tissue adjacent to cancerous location of our department between May 2004 and April 2005, which were analyzed with SP immunohistochemistry staining. Result： The positive rate of HGF were 77.5% and that of Hpa were 72.5% in laryngeal squamous cell carcinoma, which have positive correlation and were higher than that in laryngeal papilloma and normal tissue adjacent to cancerous location with significant statistical difference（P〈0.01）. The expression of HGF and Hpa had no relationship with primary locations（P〉 0.05）, that have positive correlation with clinical stages and have negative correlation with the level of cellular dif ferentiation. The expression of HGF and Hpa in lymph node metastasis group was significantly higher than the non metastasis group（P〈0.05）. Conclusion：The expression of HGF and Hpa in laryngeal quamous cell carcinoma are higher than that in nonmalignant ones. They correlates with lymph node metastasis, clinical stages and pathological grading, but they has no relationship with the primary locations. The positive correlation relationship of expression between HGF and Hpa might be worthiness to determine the prognosis of laryngeal squamous cell carcinoma.