高效液相色谱法测定广西不同产地八角果实的莽草酸含量

Determination of Shikimic Acid in Fruit of Illicium verum Hook.f. from Different Habits of Guangxi by HPLC

目的建立反相高效液相色谱法测定八角茴香中莽草酸含量的方法。方法色谱柱为Venusil HILIC（4．6mm×250mm，5μm），流动相为乙腈-0．5％三氟乙酸（95：5），流速1．0mL·min^-1，检测波长为210nm。结果平均回收率为99．54％，RSD为1．28％，符合分析要求。该方法的标准曲线为Y=33277p＋149．32，r=0．9989，线性范围为0．001～6μg。结论方法简便、结果准确、重复性好，适用于含有莽草酸的药材及制剂的质量分析。

OBJECTIVE To establish an accurate RP-HPLC method for the determination of shikimic acid in fruit ofllliciun verum Hook.f. METHODS The analytical column was Venusil HILIC column （4.6 mm×250 mm, 5 μm） , and the mobile phase consisted of acetonitrile-0.5% trifluoroacetic acid （95：5） at a flow rate of 1.0 mL·min^-1. The detection wavelength was 210 nm. RESULTS The average recovery and RSD were 99.54 % and 1.28%, respectively. The calibration curve of shikimic acid for this method was Y=33 277 ρ＋149.32 （r=0.998 9） in the range of 0.001-6 μg. CONCLUSIONS This method is simple, accurate and reproducible, and it can be used for the determination of shikimic acid in herb medicines and preparation containing shikimic acid.