固定化CGMCC No.2266生物转化法制备(S)-(-)-β-羟基苯丙酸乙酯

Preparation of (S)-(-)-β-hydroxy-benzenepropanoic acid ethyl ester by asymmetric reduction of β-carbonyl phenylpropionate with immobilized CGMCC No.2266

在邻苯二甲酸二丁酯中采用固定化酿酒酵母CGMCC No.2266不对称还原β-羰基苯丙酸乙酯制备(S)-(-)β--羟基苯丙酸乙酯。将干重为430 mg的100 ml菌液50℃预热处理30 min,以2%海藻酸钠固定化得到的直径为2 mm的固定化细胞增殖培养72 h,在300 ml邻苯二甲酸二丁酯中转化17.2 mmol.L-1β-羰基苯丙酸乙酯,摩尔产率和对映体过剩值可以分别达到92.2%和100%。固定化细胞可以较好地重复利用于转化反应10次。产率随底物浓度的增高而降低,分批加入底物可以降低底物浓度过高对反应过程的抑制。

（S） - （--） -β-hydroxy benzenepropanoic acid ethyl ester was synthesized by asymmetric reduction of β-carbonyl phenylpropionate with immobilized CGMCC No. 2266 in dibutyl phthalate. 100 ml microbial liquor containing 430 mg （dry weight） biomass preheated at 50℃ for 30 rain was immobilized. After re culturing for 72 h, the immobilized cells of 2 mm diameter prepared by 2% sodium alginate was used to reduce 17.2 mmol · L ^-1 β-carbonyl phenylpropionate in 300 ml dibutyl phthalate. The yield and enantiomeric excess of （S） - （--） -β hydroxy benzenepropanoic acid ethyl ester reached 92.20/00 and 100% respectively. Immobilized CGMCC No. 2266 can be reused in reaction for ten times. The yield decreased with increasing substrate concentration. Batch addition of substrate can reduce inhibition of high concentration substrate.