NaCl胁迫对嫁接番茄根系质膜和液泡膜ATP酶活性的影响

Effects of NaCl stress on ATPase activities of root plasma and tonoplast membranes in grafted tomato seedlings

在NaCl胁迫下,对番茄嫁接苗和自根苗的根系活力、根系质膜H^+-ATPase、液泡膜H^+-ATPase和H^+-PPase、质膜和液泡膜Ca^(2+)-ATPase、质膜氧化还原系统活性进行了比较。结果表明,胁迫条件下,嫁接苗根系活力显著高于自根苗。胁迫前期,嫁接苗根系质膜H^+-ATPase活性、液泡膜H^+-ATPase和H^+-PPase活性、质膜和液泡膜Ca^(2+)-AT- Pase活性、质膜NADH氧化速率和Fe(CN_6^(3-))还原速率被显著诱导;自根苗根系液泡膜H^+-ATPase、H^+-PPase和Ca^(2+)-ATPase活性、质膜NADH氧化速率和Fe(CN)_6^(3-)还原速率被显著诱导。胁迫后期,嫁接苗和自根苗根系各项指标均被显著抑制,但嫁接苗各指标受抑制时间较自根苗晚,且数值上均显著高于自根苗。表明嫁接苗比自根苗具有较强的耐盐性。

The effects of NaCl stress on root vigor, H^＋ -ATPase activities of plasma membrane and tonoplast membrane, H^＋-PPase activities of tonoplast membrane, Ca^2 ＋ -ATPase activities of plasma membrane and tonoplast membrane, plasma membrane redox system in roots of grafted and own-root tomato （ Lycopersicon esculentum Mill. ） seedlings were investigated. Results showed that root vigor of grafted seedlings was significantly higher than that of own-root seedlings during the whole stage of stress. In the early stage of stress, H^＋ -ATPase activities of plasma membrane and tonoplast membrane, H^＋ -PPase activities of tonoplast membrane, Ca^2＋ -ATPase activities of plasma membrane and tonoplast mem- brane, NADH oxidation rate, Fe（CN）6^3 - reduction rate in roots of grafted seedling were significantly increased, H^＋ -AT- Pase, H^＋ -PPase and Ca^2 ＋ -ATPase activities of tonoplast membrane, NADH oxidation rate, Fe（CN）3^6- reduction rate of own-root seedling roots were significantly increased as well. In the late stage of stress, the activities of all indices of both grafted and own-root seedlings were inhibited, but the inhibition of grafted seedlings was later and significantly lighter than those of own-root seedlings during the whole stage of stress. All these results indicated that the tolerance of grafted tomato seedlings to NaCl stress was stronger than that of own-root seedlings.