两所医院铜绿假单胞菌连续分离株耐药性与整合子、转座子遗传标记研究

Antibiotic-resistant Genetical Marks of Integron and Transposon in Pseudomonas aeruginosa Strains Isolated in Two Hospitals

目的了解江浙地区两所医院铜绿假单胞菌(PAE)连续分离株的耐药性与遗传标记。方法采用K-B法检测PAE连续分离株的耐药性,PCR方法检测4种整合子、转座子遗传标记(qacE△1-sul1、mert、npAt、npU)。结果两所医院PAE对头孢哌酮/舒巴坦、亚胺培南、美罗培南与阿米卡星药物敏感性尚好,对β-内酰胺类、环丙沙星、复方新诺明耐药率极高,PAE检出qacE△1-sul1基因(48.6%、45.0%)和merA(11.4%、5.0%),tnpA、tnpU未检出。结论PAE连续分离株携带qacE△1-sul1、merA基因是细菌呈多药耐药的主要原因,具有流行病学意义。

OBJECTIVE To investigate the antibiotic resistance and the genetical marks in Pseudomonas aeruginosa （PAE） strains in two hospitals of Jiangsu and Zhejiang. METHODS The drug-resistance in PAE strains was detected with K-B test, four kinds of genetical marks of integron and transposon （qacE△l-sull, mer, tnpA and tnpU） were detected by PCR. RESULTS The strains were only sensitive to cefoperazone/sulbactam, imipenem, meropenem and amikacin. There was a high resistance rate to β-1actamases, eiprofloxaein, and sulfamethoxazole. Genes of qacE△l-sull （48.6 %, 45.0%） and merA （11.4 %//00,5.0 0% ） were detected from PAE strains in the two hospitals. There were no tnpA and tnpU too. CONCLUSIONS Multiple-drug-resistance in Pseudomonas aeruginosa （PAE） strains is caused mainly by qacE△l-sull and merA.