硫酸化黄芪多糖佐剂活性的比较

Comparative study on adjuvanticity of sulfated astragalus polysaccharides

14日龄雏鸡200只,随机均分为10组,在用新城疫弱毒苗免疫的同时,6个试验组分别肌肉注射高、低剂量3种修饰的硫酸化黄芪多糖(sAPS)sAPS40、sAPS50和sAPS60,2个多糖对照组注射高、低剂量未修饰的黄芪多糖(APS),无佐剂对照组注射等量生理盐水,每天1次,连续3 d。分别于免疫后7、14、21、28 d翅静脉采血测定血清抗体效价;于免疫后14、21、28、35 d心脏采血测定T淋巴细胞增殖。结果表明,3种修饰多糖均能显著提高血清抗体效价、促进T淋巴细胞增殖,作用强于未修饰多糖,并有一定的量效和时效关系,高剂量提高血清抗体效价、低剂量促进T淋巴细胞增殖的效果较好,综合评价以sAPS60最好,可以作为新型免疫增强剂的候选药。

Two hundred 14-day-old chickens were divided randomly into 10 groups. After inoculated with ND-Ⅳ vaccine, the chickens in six experimental groups were intramuscularly injected respectively with three sulfated astragalus polysaccharides （sAPSs）, sAPS40, sAPS50 and sAPS60, at high and low dose taking non-modified APS as control, in free adjuvant control group, physiological saline, once a day for three successive days. On days 7,14,21 and 28 after vaccination,the blood samples were collected from brachial vein for determination of serum HI antibody. On days 14,21,28 and 35 after vaccination, the blood samples were collected by cardiopuncture for determination of peripheral T lymphocyte proliferation. The results showed that three modified sAPSs could significantly enhance antibody titer and promote lymphocyte proliferation, which were better than those of non-modified APS and presented the relations to dose-and time-effect. At high dose, their effects of enhancing antibody titer were the better, at low dose,promoting lymphocyte proliferation. In general evaluation the efficacy of sAPS60 was best and would be expected as a candidate of new immunopotentiator.