转基因小鼠前列腺癌及转移的三维超声微成像

The use of three-dimensional ultrasound microimaging for detecting tumor and metastasis in the transgenic mouse adenocarcinoma prostate model

目的:应用三维超声微成像检测转基因小鼠前列腺腺癌(TGMAP)模型的前列腺肿瘤和转移。方法:使用三维超声微成像系统监测TGMAP模型小鼠的前列腺肿瘤生长。通过对TGMAP小鼠前列腺癌的三维超声图像和前列腺癌标本的比较,验证该超声系统检测活体小鼠肿瘤大小的可靠性。结果:超声成像可检测到直径为2.4～14mm的肿瘤和转移。通过三维超声体内测量与尸检获得的肿瘤最大直径的相关系数为0.998,超声诊断的敏感性和特异性均>90%。结论:TGMAP模型的三维超声微成像有望成为小鼠临床前期研究的新的微成像手段。

Objective To investigate the use of three-dimensional ultrasound microimaging for detecting tumor and metastasis in the transgenic mouse adenocarcinoma prostate （TGMAP） model. Methods The three-dimensional ultrasound microimaging was used for measuring tumor progression in TGMAP model. Quantitive comparisons of three-dimensional ultrasound images with gross pathology of prostate tumors were clone to show the ability of ultrasound to depict the size of malignant masses in live mice. Results Ultrasound imaging identified tumors ranging from 2.4 to 14 mm maximum diameter. The correlation coefficient of tumor diameter measurements done in vivo with three-dimensional uhrasound and at autopsy was 0.998. Prospective tumor detection sensitivity and specificity were both 〉 90% when diagnoses were based on repeated ultrasound examinations done on separate days. Conclusion The application of three- dimensional ultrasound imaging to prostate cancer in mice will likely enable ultrasound to become a new microimaging modality for mouse preclinical trial studies.