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莱菔硫烷诱导人肝细胞硫氧还蛋白还原酶表达 被引量：2

Induction of TR1 by sulforaphane in human immortalized hepatocytes
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摘要 目的探讨莱菔硫烷(sulforaphane,SFN)对人肝细胞系HHL-5细胞硫氧还蛋白还原酶-1(thioredoxin reductase1,TR1)的诱导作用及机制。方法采用WST试验观察SFN对HHL-5细胞24h的细胞毒作用,及TaqMan one-step RT-PCR测定对TR1mRNA的诱导,蛋白印迹法(Western blotting)测定核因子E2相关因子2(nuclear factor erythroid2-related factor2,Nrf2)的蛋白表达。结果5mol/LSFN处理HHL-5细胞24h促进细胞增殖,更高剂量表现生长抑制作用;SFN处理HHL-5细胞24h呈剂量依赖性诱导TR1转录,10mol/LSFN对TR1的诱导为对照组的4.6倍;20μmol/LSFN处理1h后明显诱导了胞浆和胞核Nrf2蛋白表达,并且这种诱导呈明显的时间依赖性。结论SFN可能通过诱导转录因子Nrf2介导的TR1转录,从而增强机体抗氧化防御系统机能。 Objective To study the effect of sulforphane（SFN） on TR1 mRNA expression in human immortalized hepatocytes（HHL-5 cells） and the potential mechanism involved.Methods WST assay was used to observe the cytotoxicity of SFN.TR1 mRNA induction was quantified by TaqMan one-step RT-PCR and Nrf 2 expression was detected by Western blot.Results SFN promoted HHL-5 cells growth at 5μM and inhibited cells growth at higher dose.TR1 mRNA was induced dose-dependently by SFN（2～10μM 24h）,with the maximal induction of 4.6-fold with 10μM SFN treatment.Moreover,Nrf 2 protein was upregulated in a time-dependent manner both in cytoplasm and in nucleus after 20μM SFN exposure,and significant induction was shown at 1h.Conclusion SFN might increase antioxidative defense in cells through induction of TR1 via Nrf 2-mediated transcription.
作者 李丹 暴永平 吴坤
机构地区 哈尔滨医科大学公共卫生学院营养与食品卫生学教研室 School of Medicine
出处 《中国公共卫生》 CAS CSCD 北大核心 2009年第2期189-191,共3页 Chinese Journal of Public Health
关键词 莱菔硫烷 异硫氰酸盐 硫氧还蛋白还原酶 核因子E2相关因子2 sulforaphane（SFN） isothiocyanates（ITCs） thioredoxin reductasel（TR1） nuclear factor erythroid 2-related factor 2（Nrf 2）
分类号 R151.3 [医药卫生—营养与食品卫生学]
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参考文献12

  • 1Zhao H,Lin J,Grossman HB,et al. Dietary isothiocyanates,GSTM1, GSTT1 ,NAT2 polymorphisms and bladder cancer risk [ J ]. Int J Cancer,2007,120(10) :2208 -2213.
  • 2Rask L,Andreasson E,Ekbom B,et al. Myrosinase:gene family evolution and herbivore defence in Brassicaceae[ J]. Plant Mol Bio, 2000,42(1) :93 -113.
  • 3Zhang Y, Munday R, Jobson He, et al. Induction of GST and NQO1 in cultured bladder ceils and in the urinary bladders of rats by an extract of broccoli( Brassica oleracea italica) sprouts[ J]. J Agric Food Chem,2006,54(25 ) :9670 -9676.
  • 4侯春彦,张劲松.莱菔硫烷脱毒和抗氧化功能研究进展[J].中国公共卫生,2008,24(5):626-628. 被引量：10
  • 5Papp LV, Lu J, Holmgren A, et al. From selenium to selenoproteins : synthesis, identity, and their role in human health[J]. Antioxid Redox Signal,2007,9 (7) :775 - 806.
  • 6Svehlikova V,Wang S,Jakufkova J, et al. Interactions between sulforaphane and apigenin in the induction of UGT1A1 and GSTA1 in CaCo - 2 cells[ J]. Carcinogenesis,2004,25 ( 9 ) : 1629-1637.
  • 7Tang L, Zhang Y, Jobson HE, et al. Potent activation of mitochondria-mediated apoptosis and arrest in S and M phases of cancer cells by a broccoli sprout extract [ J ]. Mol Cancer Ther, 2006,5 (4) :935 -944.
  • 8Itoh K, Chiba T, Takahashi S, et al. An Nrf 2/small Mar heterodimer mediates the induction of phase Ⅱ detoxifying enzyme genes through antioxidant response elements [ J ]. Biochem Biophys Res Co mmun, 1997,236 (2) :313 - 322.
  • 9Itoh K,Wakabayashi N, Katoh Y,et al. Keap 1 represses nuclear activation of antioxidant responsive elements by Nrf 2 through binding to the amino-terminal Nef 2 domain [J]. Genes Dev,1999,13(1 ) :76 -86.
  • 10Kobayashi A, Kang MI, Okawa H, et al. Oxidative stress sensor Keap 1 functions as an adaptor for Cul3-based E3 ligase to regulate proteasomal degradation of Nrf 2 [ J]. Mol Cell Biol, 2004, 24(16) :7130 -7139.

二级参考文献38

  • 1Moi P,Chan K,Asunis I,et al.Isolation of NF-E2-related factor 2 (Nrf2),a NF-E2-like basic leucine zipper transcriptional activator that binds to the tandem NF-E2/AP1 repeat of the beta-globin locus control region Proc[J].Natl Acad Sci USA,1994,91(21):9926-9930.
  • 2McMahon M,Thomas N,ltoh K,et al.Redox-regulated turnover of Nrf2 is determined by at least two spearate protein domains,the redox-sensitive Neh2 degron and the redox-insensitive Neh6 degron[J].Biol Chem,2004,279(30):31556-31567.
  • 3Katoh Y,ltoh K,Yoshida E,et al.Two domains of Nrf2 cooperatively bind CBP,a CREB binding protein,and synergistically activate transription[J].Genes Cells,2001;6(10):857-868.
  • 4Kobayashi A,Ohta T,Yamamoto M,Unique function of the Nrf2-keap1 pathway in the inducible expression of antioxidant and detoxifying enzynmes[J].Methods Enzymol,2004,378:273-286.
  • 5Kobayashi M,ltoh K,Suzuki T,et al.ldentification of the interactive interface and phylogenic conservation of the Nrf2-Keap1 system[J].Genes Cells,2002,7(8):807-820.
  • 6kang Ml,Kobayashi A,Wakabayashi N,et al.Scaffolding of Keap1 to the actin cytoskeleton controls the function of Nrf2 as key regulator of cytoprotective phase 2 genes[J].Proc Natl Acad Sci USA,2004,101(7):2046-2051.
  • 7Zipper LM,Mulcahy RT.The Keap1 BTB/POZ dimerization function is required to sequester Nrf2 in cytoplasm[J].Biol Chem,2002,277(39):36544-36552.
  • 8Dinkova Kostova AT,Holtzclaw WD,Cole RN,et al.Direct evidence that sulfhydryl groups of Keap1 are the sensors regulating induction of phase 2 enzymes that protect against carcinogens and oxidants[J].Proc Natl Acad Sci USA,2002,99(18):11908-11913.
  • 9Zhang DD,Hannink M,Distinct cysteine residues in Keap1 are required for Keap1-dependent ubiquitination of Nrf2 and for stabilization of nrf2 by chemopreventive agents and oxidative stress[J].Mol Cell Biol,2003,23(22):8137-5151.
  • 10Ltoh K,Wakabayashi N,Katoh Y,et al.keap1 regulates both cytoplasmic-nuclear shuttling and degradation of Nrf2 in response to electrophiles[J].Genes Cells,2003,8(4):379-391.

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中国公共卫生
2009年 第2期

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