RP-HPLC法同时测定不同产地及不同部位防风中4种有效成分的含量

Content determination of four components from different habitats and different parts of Saposhnikovia divaricata(Turcz.)Schischk by RP-HPLC

目的建立防风药材中4种有效成分含量同时测定的方法，以比较不同产地防风药材及不同部位中有效成分的含量，为防风药材质量标准的制定及防风的资源开发提供科学依据。方法采用RP-HPLC法。色谱柱：Diamonsil C18柱（200mm×4．6mm，5μm）；流动相：甲醇-水，梯度洗脱；流速：1．0mL·min^-1；检测波长：254mm；柱温：30℃。结果升麻素、升麻素苷、5-O-甲基维斯阿米醇苷、亥茅酚苷能够达到基线分离；升麻素苷和5-O-甲基维斯阿米醇苷质量浓度在1．92～400mg·L^-1内与峰面积呈良好的线性关系（r=0．9999、0．9998，n=5），平均加样回收率分别为97．5％、97．7％，RSD分别为1．3％、2．8％（n=9）；升麻素、亥茅酚苷质量浓度在0．96～200mg·L^-1内与峰面积呈良好的线性关系（r=0．9999、0．9999，n=5），平均加样回收率分别为99．3％、97．7％，RSD分别为2．0％、1．7％。结论本方法为防风药材的资源开发提供了参考，可用于防风药材的质量控制。

Objective A high performance liquid chromatographic method was established for analysis of four components from different habitats and different parts of Saposhnikovia divaricata （Turcz.） Schischk. Methods Diamonsil C18 column（ 200 mm × 4.6 mm ,5 μm）was used with a mixture of methanol and water as the mobile phase, gradient elution was performed. The flow rate was 1.0 mL. min^-1, detection wavelength was set at 254 nm, column temperature was set at 30℃. Results The linear range of prim-O-glucosylcimifugin,4＇-O-β-D-glucosyl-5-O-methylvisamminol and cimifugin, sec-O-glucosylhamaudol were 1.92 - 400 mg. L^-1 and 0.96-200 mg.L^-1, respectively. The average recoveries of prim-O-glucosylcimifugin and 4＇-O-β-D- Glucosyl-5-O-methy-lvisamminol were 97.5% and 97.7% with RSD of 1.3% and 2.8% ,respectively. The average recoveries of cimifugin and sec-O-glucosylhamaudol were 99.3% with RSD of 2.0% ,97.7% with RSD of 1.7 %, respectively. Conclusions The method is simple, accurate and sensitive with good reproducibility. It can provide references for quality control and resources development of the medicinal materials.