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在无血清条件下TNFα通过Pkn1和MAPK-Erk通路对FBJ细胞生长的调控作用 被引量：6

Regulation of cell survival of mouse osteosarcoma FBJ cells by TNFα via Pkn1 and MAPK-Erk signals in serum free medium
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摘要 目的研究肿瘤坏死因子α(tumor necrosis factorα,TNFα)在无血清条件下作为生长因子促进FBJ细胞生长的信号通路。方法用细胞活性检测法分析细胞的活性;分别用逆转录聚合酶链式反应(reverse transcriptional-polymerase chain reaction,RT-PCR)法和免疫印迹法检测细胞中TNFα的表达以及胞外信号调节激酶(extracellular signal-regulated kinase,Erk)、p38有丝分裂原活化蛋白激酶(p38 mitogen-activated protein kinase,p38 MAPK)的磷酸化水平。结果在无血清培养基培养条件下,经TNFα(10μg.L-1)处理的FBJ细胞明显对抗无血清条件下诱发的细胞死亡,TNFαsense cD-NA转染的FBJ-LL和FBJ-S1细胞均能提高其在正常培养基以及无血清培养基中的生长速度,TNFα是FBJ细胞中重要的生长因子之一;蛋白酶N1(protein kinase N1,Pkn1)siRNA可明显沉默目的基因Pkn1的表达,同时降低TNFα的含量;Pkn1沉默的细胞在无血清条件下的生长明显被抑制;TNFα处理的细胞能迅速刺激Erk的磷酸化,Erk沉默的FBJ-LL细胞的生长速度明显降低,TNFα通过Erk信号通路诱发细胞生长;TNFα亦能诱导另一有丝分裂原活化蛋白激酶(mitogen-ac-tivated protein kinase,MAPK)家族p38的磷酸化;神经节苷脂GD1a可降低TNFα在FBJ细胞中的表达,TNFα诱发的无血清条件下的生长速度也被神经节苷脂GD1a抑制。结论在无血清条件下,TNFα通过Pkn1和MAPK-Erk通路调控小鼠骨肉瘤FBJ细胞的生长。 Objective To verify the roles of tumor necrosis factor α（TNFα） as a growth factor in FBJ cells growth in serum free medium. Methods Cells viability was detected by tetrazolium assay. Expression of TNFα and activation of extracellular signal-regulated kinase （Erk）, p38 were determined by reverse transcriptional polymerase chain reaction or western blotting, respectively. Results TNFα addition to FBJ-LL and FBJ-S1 cells significantly enhanced cell survival in serum free medium, transfection of FBJ-LL and FBJ-S1 cells with TNFα sense cDNA markedly enhanced cell survival in serum free medium. SiRNA of Pknl suppressed TNFα as well as Pknl ,in serum free medium,cell survival of protein kinase N 1 silenced cells was suppressed and increased by treatment of TNFα, stimulation of FBJ-LL cells with TNFα increased phospho- rylation of MAPK-Erk rapidly and significantly, silence of Erk by siRNA resulted in cell death comparing to scrambled transfected cells. TNFα has been proved to be suppressed by ganglioside GDla. In this study,it was also found that TNFα enhanced cell growth in serum free medium was suppressed by the presence of GDla. Conclusions TNFα is responsible for cell survival of FBJ cells in serum free medium through Pknl and MAPK-Erk signal pathways.
作者 王丽 王毅楠 吴英良 山形贞子 山形达也
机构地区 沈阳药科大学生命科学与生物制药学院
出处 《沈阳药科大学学报》 CAS CSCD 北大核心 2009年第2期145-151,共7页 Journal of Shenyang Pharmaceutical University
关键词 肿瘤坏死因子α 胞外信号调节激酶 蛋白酶N1 FBJ细胞 细胞死亡 神经节苷脂 GD1a tumor necrosis factor α （TNFα） extracellular signal-regulated kinase （Erk） protein kinase N1 （Pkn1） FBJ cell cell death ganglioside GD1a
分类号 R963 [医药卫生—微生物与生化药学]
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参考文献12

  • 1LOCKSLEY R M, KILLEEN N, LENARDO M J. The TNF and TNF receptor superfamilies: Integrating mammalian biology [J]. Cell, 2001,104 ( 4 ) : 487 - 501.
  • 2VAN HORSSEN R, TEN HAGEN T L, EGGEMONT A M. TNF-alpha in cancer treatment: Molecular insights, antitumor effects, and clinical utility [ J ]. Oncologist,2006,11 (4) : 397 - 408.
  • 3WU S,BOYER C M,WHITAKER R S,et al. Tumor necrosis factor alpha as an autocrine and paracrine growth factor for ovarian cancer: Monokine induction of tumor cell proliferation and tumor necrosis factor alpha expression [ J ]. Cancer Res, 1993, 53 ( 8 ) : 1939 - 1944.
  • 4GAIOTTI D, CHUNG J, IGLESIAS M, et al. Tumor necrosis factor-alpha promotes human papillomavirus (HPV) E6/E7 RNA expression and cyclin-dependent kinase activity in HPV-immortalized keratinocytes by a ras-dependent pathway [ J 3. Mol Carcinog, 2000127 (2) :97 - 109.
  • 5MAEDA M, WATANABE N, OKAMOTO T, et al. Endogenous tumor necrosis factor functions as a resistant factor against adriamycin [ J ]. Int J Cancer, 1994,58 (3) :376 - 379.
  • 6MURAKAMI A, KAWABATA K, KOSHIBA T, et al. Nitric oxide synthase is induced in tumor promotersensitive, but not tumor promoter-resistant, JB6 mouse epidermal ceils cocultured with interferon-gammastimulated RAW264.7 cells: The role of tumor necrosis factor-alpha [ J ]. Cancer Res, 2000, 60 ( 22 ) : 6326 - 6331.
  • 7ORTON R J, STURM O E, VYSHEMIRSKY V, et al. Computational modelling of the receptor-tyrosine- kinase-activated MAPK pathway [ J ]. Biochem J, 2005,392(2) :249-261.
  • 8RIVAS M A, CARNEVALE R P, PROIETTI C J, et al. TNF alpha acting on TNFR1 promotes breast cancer growth via p42/P44 MAPK, JNK, Akt and NF-kappa B-dependent pathways[J].Exp Cell Res,2008,314 (3) :509 -529.
  • 9WANG Li, WANG Yi-nan, SATO T, et al. Ganglioside GDla suppresses TNFα expression via Pknl at the transcriptional level in mouse osteosarcoma-delived FBJ cells [ J ]. Biochem Biophys Res Commun, 2008,371 (2) :230 - 235.
  • 10YAMAGATA S, MIWA M, TANAKA K, et al. FBJ virus-induced osteosarcoma has type V collagen consisting of A,B and C-like chains in addition to type I collagen [J]. Biochem Biophys Res Commun, 1982, 105(3) :1208 - 1214.

二级参考文献13

  • 1HYUGA S, YAMAGATA S, TAKATSU Y, et al. Suppression of FBJ - LL cell adhesion to vitronectin by ganglioside GDla and loss of metastatic capacity[J]. International J Cancer, 1999, 83:685 -691.
  • 2WANG L, TAKAKU S, WANG P, et al. Gangfioside GDla regulation of caveolin - 1 and stiml expression in mouse. FBJ cells: Augmented expression of caveolin- 1 and Stiml in cells with increased GDla content [J]. Glycoconj J, 2006, 23 : 303 - 315.
  • 3HU D, TAN X, SATO T, . et al. Apparent suppression of MMP- 9 activity by GDla as deterrnined by gelatin zymography [J]. Biochern and Biophys Res Commun, 2006, 349:426 - 431.
  • 4YAMAGATA S, TANAKA R, ITO Y, et al. Gelatinase of murine metastatic tumor cells [J]. Biochem Biophys Res Commun, 1989, 158:228 - 234.
  • 5ISHIYAMA M, SHIGA M, SASAMOTO K, et al. A new sulfonated tetrazolium salt that produces a highly watersoluble formazan dye [ J ]. Chem Pharm Bull ( Tokyo ), 1993, 41:1118 - 1122.
  • 6ROZEN S, SKALETSKY H J. Primer3 on the WWW for general users and for biologist programmers [ M ]. Totowa, NJ: Humana Press, 2000 : 365 - 386.
  • 7BRATTON DL, FADOK VA, RICHTER DA, et al. Appearance of phosphatidylserine on apoptotic cells requires calcium- mediated nonspecific flip - flop and is enhanced by loss of the aminophospholipid translocase [ J ]. J Biol Chem, 1997,272.26 159-26 165.
  • 8RODECK U. Growth factor independence and growth regulatory pathways in human melanoma development [ J ].Cancer Metastasis Rev, 1993, 12:219 - 226.
  • 9BHUNIA AK, SCHWARZMANN G, CHATTERJEE S, et al. GD3 recruits reactive oxygen species to induce cell proliferation and apoptosis in human aortic smooth muscle cells [J] .J Biol Chem, 2002, 277:16 396- 16 402.
  • 10HA KT, LEE YC, KIM CH, et al. Overexpression of GD3 synthase induces apoptosis of vascular endothelial ECV304 cells through downregulation of Bcl - 2 [ J ]. Febs Lett, 2004, 568:183 - 187.

同被引文献28

  • 1Yang Zhao,Jing-Wen Wang,Tsutomu Tanaka,Akihiro Hosono,Ryosuke Ando,Shinkan Tokudome,Soeripto,FX Ediati Triningsih,Tegu Triono,Suwignyo Sumoharjo,EY Wenny Astuti Achwan,Stephanus Gunawan,Yu-Min Li.Association between TNF-a and IL-1β genotypes vs Helicobacter pylori infection in Indonesia[J].World Journal of Gastroenterology,2013,19(46):8758-8763. 被引量：5
  • 2Hidalgo. Calcium,iron and neuronal function[J].IUBMB Life, 2007,59(4/5) ;280-285.
  • 3de Bernardo S. Role of extracellular signal-regulated protein ki nase in neuronal cell death induced by glutathione depletion in neuron/gliames encephalic cultures[J]. J Neuroehem, 2004,91 (3) : 667-682.
  • 4Lu KT. NK CCl-mediated traumatic brain injury induced brain edema and neuron death via Raf / MEK/ MAPK cascade [J]. Crit Care Med,2008,36(3) :917-922.
  • 5Zhang JZ. Inhibitory effect of ketamine on phosphorylation of the extracellular signal regulated kinase 1/2 following brain is- chemia and reperfusion in rats with hyperglycemia[J]. Exp Toxicol Pathol,2007,59(3 4) :227-235.
  • 6Luo Y. Opposing roles for ERK1/2 in neuronal oxidative toxici ty:distinct mechanisms of ERK1/2 action at early versus late phases of oxidative stress[J].Biol Chem, 2006,281 (24) : 16 436-16 442.
  • 7FURUKAWA Y, OKUYAMA S,AMAKURA Y,etal.Isolation and characterization of activators of ERK/MAPK from citrus plants[J].Int J Mol Sci,2012,13(2):1832-1845.
  • 8VAN HORSSEN R,TEN HAGEN TL,EGGEMONT AM.TNF-al- pha in cancer treatment:Molecular insights,antitumor effects,and clinical utility[J].Oneologist,2006,11(4):397-408.
  • 9Dent P,Yacoub A,Fisher PB,etal.MAPK pathways in radiation re- sponses[J].Oncogene,2003,22(37):5885-5896.
  • 10Dziareke R,Jin YP,Gupta D.Differential activation of extraeellular Signal-regulated kinase (EBX)l,ERK2,P38 and e-Jun N-terminal kinase mitogen-activated protein kinases bybacterial peptidoglycan.J Infect Dis, 1996,174(4):777-785.

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沈阳药科大学学报
2009年 第2期

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