大鼠创伤性休克早期血液单个核细胞释放HSP70的变化及甘氨酸的影响

Effects of glycine on the release of heat shock protein 70 from blood mononuclear cells of rats with taumatic shock

目的研究观察血液单个核细胞(blood mononuclear cells,BMCs)释放HSP70、TNF-α和IL-6水平的动态变化及甘氨酸的干预影响。方法建立创伤性休克动物模型,88只Wistar大鼠随机分成3组:治疗组(T组)、休克组(S组)和对照组(C组)。在复苏开始时,T组大鼠经颈静脉输入甘氨酸液0.5mL(甘氨酸浓度为100mg/kg的生理盐水);S组输以相同体积的生理盐水;C组仅行腹腔麻醉。分别于复苏后3、6、12和24h共4个时相点活杀大鼠。采用酶联免疫吸附法(ELISA)检测BMCs释放HSP70、TNF-α和IL-6的水平。结果S组大鼠复苏后3h BMCs释放HSP70、TNF-α和IL-6水平均出现升高,复苏后6h达高峰。与S组相比,T组大鼠各个时间点BMCs释放HSP70水平均明显增高,而释放TNF-α和IL-6水平均明显降低(均P<0.01)。结论甘氨酸可促进BMCs释放HPS70、抑制BMCs释放TNF-α、IL-6,可望起到防治创伤性休克后SIRS及MODS的作用。

[Objective] To investigate the effects of glycine on the release of HSP70, TNF-α and IL-6 from blood mononuclear cells （BMCs） in rats with traumatic shock. [Methods] The traumatic shock model was established and 88 Wistar rats were divided randomly into three groups： treatment group （T group）, shock group （S group） and control group （C group）. At the beginning of resuscitation, the rats in T group received 0.5 mL normal saline contained 100 mg/kg glycine by external jugular vein, those in the shock group received only 0.5 mL normal saline. And the rats in the C group received only intrapefitoneal anesthesia. The rats in the three groups were killed at 3, 6, 12 and 24 h after resuscitation respectively. The levels of HSP70, TNF-α and IL-6 released from BMCs were detected by ELISA. [Result] The levels of of HSP70, TNF-α and IL-6 released from BMCs of rats in S group increased at 3 h and reached the peak value at 6 h after resuscitation. Compared with S group, the level of HSP70 in BMCs in T group increased significantly and the levels of TNF-α and IL-6 decreased significantly （all P 〈0.01）. [Conclusion] Glycine can promote the release of HSP70 and reduce the release of TNF-α and IL-6 from BMCs after traumatic shock. It may play an important role in the prevention and treatment of the secondary SIRS and MODS after traumatic shock.