VEGF降低家兔脑缺血再灌注损伤与caspase-3表达的关系

VEGF alleviates cerebral ischemia/reperfusion injury and the results in expression of caspase-3 in rabbits

目的探讨血管内皮生长因子(VEGF)治疗家兔局灶性脑缺血/再灌注损伤与半胱氨酸蛋白酶-3(caspase-3)表达的关系。方法复制兔局灶性脑缺血/再灌注模型,分别在缺血2 h,再灌注0、1和3 h应用微量进样器将VEGF立体定向导入梗死灶周,于再灌注3 d观察神经功能、梗死体积、水肿体积、灶周凋亡数和caspase-3表达。结果缺血/再灌注0和1 h时,灶周给予VEGF,梗死体积分别较对照组下降23.3%、17.9%,相应的水肿体积、灶周凋亡率及caspase-3表达明显下降(P<0.01),神经功能恢复较好,用药越早越明显;再灌注3 h后给药,则无明显作用。梗死体积下降与caspase-3表达下降明显相关(P<0.05)。结论VEGF可能通过抑制caspase-3的表达减少脑缺血/再灌注损伤细胞凋亡的发生。

Objective To identify the relationship of decreased focal cerebral ischemia/reperfusion injury by VEGF in rabbits and the expression of cysteinyl aspirate-specific proteinase-3 （caspase-3） protein. Methods Focal cerebral ischemia/reperfusion model was induced. The VEGF was administered at 0, 1 and 3 h after reperfusion respectively. Infarct and edema volumes, neurological deficit score, apoptotic cells, and expression of caspase-3 were assessed at 3 d after reperfusion. Results VEGF reduced infarct volumes as compared with controls by 23.3% and 17. 9% when administered VEGF at 0 and 1 h after reperfusion. Edema volumes, apoptotic cells and expression of caspase-3 in the perifocal regions were significantly lower than that of control group when administered VEGF within 1 h after reperfusion （P 〈 0. 01 ）. No difference was found when administered VEGF at 3 h after reperfusion. Infarct volumes were significantly correlated with expression of caspase-3 in the perifocal regions （P 〈 0. 05）. Conclusion The effect of VEGF on the expression of caspase-3 is related with the neuroprotective time window. The VEGF can reduce the incidence of neuronal apoptosis during the cerebral ischemia/reperfusion injury by reducing the expression of caspase-3 protein.