糖尿病大鼠胰腺差异表达蛋白质组分析

Differential proteomic analysis of the pancreas from diabete rat

目的运用蛋白质组学方法,对糖尿病大鼠胰腺蛋白质的表达进行分析。方法用链脲佐菌素(STZ)诱发建立1型糖尿病大鼠模型。用双向凝胶电泳(2D-PAGE)分离大鼠胰腺组织蛋白质,图像分析软件对比分析大鼠的双向电泳图谱,差异表达蛋白经胶内酶切,用基质辅助激光解吸电离飞行时间质谱(MALDI-TOF)分析获得肽质量指纹图,使用Mascot方法进行检索鉴定。结果对11个差异表达蛋白质进行分析鉴定,其中4个蛋白质得到有意义结果。结论糖尿病大鼠胰腺蛋白质组的分析,有助于探索糖尿病的发病机制。

Objective To find diabetogenic mechanism by the protein profiling of the pancrea of diabete rat. Methods The rat model of type 1 diabetes was acquired by STZ administration to Wistar rat, the protein profiling of pancrea was conducted by two-dimensional electrophoresis. Then comparative imaging analysis was conducted between diabetes model and control. In-gel digestion and MALDI-TOF-MS analysis were done to get peptide mass fingerprint（PMF）. Mascot was used for the identification of proteins. Results Totally 11 diferentially-expressed protein spots were excised for MS identification, and 4 proteins were identified. Conclusion The differential analysis of pancreas proteins between the diabete rat and control are helpful for the insight into the pathogenesis of Diabetes.