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老年大鼠组织mRNA分析中内参基因的选择 被引量：2

Reference genes selection in mRNA analysis of aging rat tissues
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摘要 目的探讨用于老年大鼠组织基因mRNA表达分析的内参基因选择。方法用实时反转录PCR技术评价5个管家基因3-磷酸甘油醛脱氢酶(G3pd)、β-肌动蛋白(ACTB)、H3组氨酸3B(H3f3b)、酸性核糖体磷蛋白P0(Arbp)和18S核糖体RNA(18S)的表达水平。结果老年大鼠肾组织中表达最稳定的是管家基因ACTB,心脏和肺组织中表达最稳定的是G3pd;而Arbp在不同组织之间的表达差异最小。结论老年大鼠组织间基因表达mRNA分析应使用至少两个参照基因:一个是核糖体基因18S,另一个适合的内参基因是Arbp。 Objective To investigate the proper inner control genes suitable for mRNA expression level comparison of aging rat tissues. Methods Real-time reverse transcription PCR was used to examine in aging rat tissues the expression level of G3pd （ glyceraldehyde-3-phosphate dehydrogenase ） , ACTB （β-actin ） , H3f3b （ H3 histone, family 3 B ） ,Arbp （ acidic ribosomal phosphoprotein P0 ） and 18S （ 18 S ribosomal RNA）. Results The most stably expressed housekeeping gene in aging rat kidney was ACTB, in heart and lung G3pd showed the minimum variation; Arbp expression was the most stable one in different tissues. Conclusion For aging rat intra-tissue mRNA normalization at least two housekeeping genes should be used： one is the ribosomal RNA gene 18S and another one is Arbp.
作者 叶文静 赵晓民 侯配强 蔡洪信 夏作理
机构地区 泰山医学院生命科学研究所 泰安市疾病控制中心
出处 《基础医学与临床》 CSCD 北大核心 2009年第1期82-84,共3页 Basic and Clinical Medicine
关键词 管家基因 内参基因 实时反转录PCR MRNA HKG reference genes real-time reverse transcription PCR mRNA normalization
分类号 R346 [医药卫生—基础医学]
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参考文献12

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二级参考文献49

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共引文献134

同被引文献13

  • 1陈凤花,王琳,胡丽华.实时荧光定量RT-PCR内参基因的选择[J].临床检验杂志,2005,23(5):393-395. 被引量：58
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基础医学与临床
2009年 第1期

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