裸鼠模型的人钠/碘转运体基因转染人大细胞肺癌介导放射性核素显像

Radionuclide imaging of large cell lung cancer in nude mice mode by transfected human sodium/iodide symporter gene

目的在动物体内实验观察人钠/碘转运体(hNIS)基因转染人大细胞肺癌介导放射性核素显像是否可行。方法①利用重组质粒以脂质体转染法将hNIS基因转染入人大细胞肺癌H460细胞系中,获得稳定表达hNIS的细胞株(hNIS-H460)。②用hNIS-H460细胞株建立大细胞肺癌荷瘤裸鼠模型,进行放射性核素99mTcO4-显像和131I显像。结果①体外实验表明hNIS-H460细胞株可以摄取碘。②裸鼠大细胞肺癌(hNIS-H460)移植瘤的99mTcO4-和131I显像结果较清晰。结论转染后的hNIS-H460细胞具有一定的摄碘能力。裸鼠大细胞肺癌(hNIS-H460)移植瘤可以进行99mTcO4-和131I显像;99mTcO4-显像的质量优于131I显像。

Objective To investigate the possibility of transfecting human sodium/iodide symporter （hNIS） gene into hu man large cell lung cancer cell line H460 to facilitate radioactive nuclide imaging in vivo. Methods （1）hNIS gene was transfectted into human large cell lung cancer cell line H460 by recombinant expression ptasmids with lipofectamine 2000 plasmid complexes. Subsequently the biologic functions of stably expressing hNIS gene cell line （hNIS-460） were investigated. （2） Large cell lung cancer nude mice model was established, and the radioactive isotopes ^99mTcO1^- and ^131I imaging were performed in nude mice. Results （1）The cell line hNIS-H460 could uptake ^125I. The uptake of ^125I was 50.97 times higher in cells hNIS-H460 than in cells H460 （t=12.36, P%0.01）. （2）Radioactive isotopes 99mTeO4 and ^131I imaging in xenografts of transfected group were successfully performed using ECT imaging system. Conclusion The transfected cells hNIS-H460 can uptake iodide, and the xenografts of which can be imaged in ^99mTcO4^- and ^131 I. The quality of ^99mTcO4 imaging is better than ^131 I imaging.