流式细胞术结合组织学方法对柄海鞘血细胞的分类

Classification of Haemocytes in Styela clava by Flow Cytometry and Histology Method

用流式细胞术研究柄海鞘(Styela clava)血细胞的分类,依据细胞大小及颗粒的复杂程度将血细胞分为5类:即类群R1～R5,各类群分别占血细胞总数的37.15%±1.01%、15.85%±2.91%、16.15%±1.58%、23.65%±3.05%、5.87%±0.31%,上机血样品的密度约106个/ml。组织学方法依据细胞大小、所含颗粒的情况及细胞的染色特征将血细胞分为5类:成血细胞、透明细胞、嗜碱性颗粒细胞、嗜酸性颗粒细胞及吞噬细胞。对两种方法各自所分5类细胞的对应关系进行了讨论。

The classification of haemocytes in Styela clava was studied with flow cytometer. On the basis of cell size and presence of granules, five types of haemocytes were recognized： R1 is the smallest with a few granules; R2 is bigger with fewer granules; R3 is the midium-sized cells with more granules; R4 is the midium-sized with a few granules ; Rs is the largest ceils with more granules. The total amount of haemocytes on the average was about I x 106 cells/ml of haemolymph. The R1 , R2, R3, R4 and R5 cells constituted 37.15 % ±1.01%, 15.85 % ±2.91%, 16.15%± 1.58%, 23.65% ±3.05% ,5.87%±0.31% of the total haemocytes, respectively. Histology study revealed that haemocytes could be divided into haemoblast, hyaline cells, eosinophilic granulocytes, basophilic granulocytes and phagocytes according to cell size and the presence of granules. We also discussed the relations among the cells.