乌梢蛇卵泡不同发育期颗粒细胞的显微结构变化

Microstructural Changes on Granulosa Cells during Follicular Development in Zaocys dhumnades

采用光学显微镜主要观察了乌梢蛇(Zaocys dhumnades)卵泡发育过程中颗粒细胞的显微结构变化。结果表明,乌梢蛇的滤泡前体细胞在形态、大小与嗜色性上均与生殖基的表面上皮相似;在原始卵泡期,卵母细胞周围的滤泡前体细胞围绕成一圈;在颗粒层细胞期的时期Ⅰ至时期Ⅲ,颗粒细胞分化为由小细胞、梨形细胞与中间型细胞构成的异型颗粒细胞;在时期Ⅳ,这些异型颗粒细胞又转变成为只有小细胞的同型颗粒细胞。乌梢蛇卵泡颗粒细胞来源于生殖基的表面上皮,其发育特征是首先由同型发育成为异型,再由异型转变为同型颗粒细胞,具有同源异型的特征。

The microstructure of granulosa cells during follicular development in Zaocys dhumnades was observed. The results showed that prefollicle cells were similar to the free surface epithelium of germinal bed in morphology, size and acidophilia/basophilla. At primordial follicle stage oocytes were surrounded by prefollicle cells. At granulosa cell stage, prefollicle cells transformed into small-size cells. At stage Ⅲ, granulosa cells differentiated into three kinds of heterogeneous granulose cells which are different in morphology. At stage Ⅳ, the heterogeneous granulosa cells transformed into small-size homogeneous granulosa cells. In summary, the granulosa cells are derived from free surface epithelium of germinal bed, and homogeneous granulosa cells develop into heterogeneous ones, then back into homogeneous ones during follicle development in Z. dhumnades.