摘要
为了探讨促性腺激素释放激素类似物(gonadotropin releasing hormone analogue,GnRH-A)对动物免疫去势的效果及作用机理。在30只日本大耳白兔(Oryctolagus cuniculus)(EG-Ⅰ与EG-Ⅱ组)的颈背部皮下分2～3点注射1.0ml(100μg/ml)自制的GnRH-A抗原乳剂,EG-Ⅱ组3周后加强免疫一次;测定睾丸长度与重量、体重、血清GnRH抗体效价及睾酮浓度。结果表明,EG-Ⅱ组睾丸长度与EG-Ⅰ组差异极显著(P<0.01);EG-Ⅱ组的GnRH抗体水平明显高于EG-Ⅰ组;EG-Ⅱ组、EG-Ⅰ组与对照组的血清睾酮浓度差异逐渐增加,102d时EG-Ⅱ组睾酮浓度极显著低于对照组(P<0.01),且第28d后EG-Ⅱ组睾酮浓度显著低于EG-Ⅰ组(P<0.05);EG-Ⅱ组体重和日增重最大,显著高于EG-Ⅰ组及对照组(P<0.05)。GnRH-A主动免疫家兔对睾丸发育、血清GnRH抗体效价和睾酮浓度具有显著的影响,加强免疫效果更理想。
To investigate the efficiency of immunocastration by using gonadotropin releasing hormone analogue （ GnRH- A） in rabbits,GnRH-A was combined with bovine serum albumin （BSA） as well as incomplete Freund＇s adjuvant to make antigen emulsion. A total of 30 rabbits （ Oryctolagus cuniculus ） were injected with 1.0 ml（ 100μg/ml） GnRH-A antigen emulsion at 2 to 3 spots in two groups, EG-Ⅰ and EG- Ⅱ groups. The EG- Ⅱ group was reinjected with the same dosage after 3 weeks. The testis length and weight as well as body weight of the immunized rabbits were measured, and GnRH antibody titer and testosterone concentration were detected by ELISA. There was a significant difference in the testis length between EG- I and EG- Ⅱ groups （ P 〈 0.01 ） ; antibody level of EG-Ⅱ group was obviously higher than that of EG- I group; serum testosterone concentration in both EG- I group and EG- Ⅱ groups differed remarkably from 7 to 16 weeks when compared with tile control （ P 〈 0.05 ） ; testosterone concentration in EG- l] group was lower than that in control group （CG） at 102 d（ P 〈 0.01 ）, and testosterone concentration in EG- I group was also lower at 28 d（ P 〈 0.05） after the injecting GnRH-A antigen. Body weight and average daily body weight gain in EG-Ⅱ group were significantly higher than in EG- I and CG groups （ P 〈 0.05）. In summary, the testis development, serum GnRH antibody titer and testosterone level in experimental rabbits change obviously after active immunization with GnRH-A and an additional injection strengthenes castration effects.
出处
《动物学杂志》
CAS
CSCD
北大核心
2009年第1期139-144,共6页
Chinese Journal of Zoology
基金
国家民委2002年度重点资助项目(No.MW2002-ZD-010)
