扫描电镜观察高功率微波辐照后大鼠小肠的形态学改变

Morphologic changes of small intestines in rats after exposure to high power microwave observed with scanning electron microscope

目的:利用扫描电镜观察高功率微波(HPM)辐照后大鼠小肠形态结构的变化.方法:雄性SD大鼠35只随机分为对照组和试验组,在源距4.5 m,场强100 kV/m,总脉冲数40万次微波辐射后3,24,72 h及7 d活杀大鼠,取小肠组织;甲醛固定制备光镜标本;30 mL/L戊二醛固定,进行常规扫描电镜标本制备.结果:扫描电镜观察对照组和HPM辐照后3 h小肠形态基本正常,小肠绒毛排列整齐.辐照后24～72h,主要以肠黏膜破坏、肠上皮细胞水肿和分泌物增多为主.辐照后7 d,分泌物减少,肠绒毛水肿减轻.结论:说明HPM辐照后扫描电镜观察大鼠小肠随作用时间的不同有不同程度的损伤.

AIM： To observe the morphologic changes of small intestines in rats after exposure to high power microwave （HPM）. METHODS： Thirtyfive male SD rats were divided into control and experimental groups. The rats in experimental group were exposed to HPM at 100 kv/m for 4 - 10^5 pulses. At different time points （3 h, 24 h, 72 h and 7 d） after exposure to HPM, rats were sacrificed. The samples were fixed in 30 g/L glutaraldehyde. The morphology of small intestines was observed under scanning electron microscope （SEM）. RESULTS ： At 3 h after exposure to HPM, small intestinal villi arranged regularly as those in control group. At 24 -72 h after exposure to HPM, destroyed intestinal mucosa, swelled intestinal columnar epithelium and increased secretion were observed. At 7 d after exposure to HPM, secretion and edema of the intestinal villi reduced. CONCLUSION： Scanning electron microscopy demonstrates that high power microwave exposure at different time may cause different degrees of damage in the morphologic structures in rats.