摘要
目的:研究脂肪极基质干细胞移植对大鼠脑出血模型运动功能的影响.方法:体外培养扩增的ADSCs于造模成功后24h立体定移植入大鼠右侧脑室.移植后3,7,14,21,28d通过longa评分观察大鼠运动功能情况.各组大鼠按时处死取脑制作切片,利用免疫荧光观察ADSCs分布情况,利用凋亡染色观察各组出血灶周围凋亡细胞情况.结果:移植的细胞自侧脑室及针道逐渐向损伤灶迁移.凋亡细胞主要位于出血灶周围.移植组在7,14,21,28 d有更好地神经功能评分(P<0.05),在各时间点移植组凋亡细胞明显少于对照组(P<0.05).结论:脂肪基质干细胞移植可能通过抑制病灶周围细胞凋亡进而改善运动功能.
AIM： To explore the effect of adipose-derived stem cells （ADSCs） transplantation on motor function in intracerebral hemorrhage rats. METHODS： ADSCs were cultured and proliferated in vitro. After the rats underwent intracerebral hemorrhage for 24 h, ADSCs were stereotaxically transplanted into the right lateral ventricles. The motor function was evaluated at 3, 7, 14, 21 and 28 d after transplantation. Rats in each group were euthanized on time and brain sections were made. Immunohistochemistry was employed to observe the distribution of transplanted cells and TUNEL assay was conducted for apoptosis detection. RESULTS： The transplanted cells migrated gradually from ventricles to the vicinities of hemorrhage focus and the hole of needle. The majority of apoptotic cells distributed around the focus of hemorrhage. Compared with the control, ADSCs-transplanted group had better motor function at 7, 14, 21 and 28 d （ P 〈 0.05 ） and fewer apoptotic cells at 3, 7, 14, 21 and 28 d after transplantation （ P 〈 0.05 ）. CONCLUSION ： ADSCs transplantation may improve the motor function by reducing apoptotic cells in intracerebral hemorrhage rats.
出处
《第四军医大学学报》
北大核心
2009年第3期236-239,共4页
Journal of the Fourth Military Medical University
关键词
脂肪基质干细胞
脑出血
细胞凋亡
adipose-derived stem cells
intracerebral hemorrhage
apoptosis
