甲基丙烯酸环氧丙酯致人支气管上皮恶性转化细胞DNA修复基因点突变的研究

Point Mutation of DNA Repair Genes of Human Bronchial Epithelial Cells Malignant Transformation Induced by Glycidyl Methacrylate

背景与目的:研究甲基丙烯酸环氧丙酯(glycidyl methacrylate,GMA)致人支气管上皮(16HBE)恶性转化细胞DNA修复基因点突变情况。材料与方法:采用聚合酶链式反应-限制性片段长度多态性方法(PCR-RFLP)检测GMA致16HBE恶性转化细胞DNA修复基因hMSH2、XRCC1、XPD及XRCC3的重要位点的突变情况,并以DNA测序方法加以验证。结果:16HBE细胞hMSH2IVS12-6(T>C)位点发生了突变,由野生基因型TT型突变为TC基因型,其它位点未检测到突变。DNA测序结果相符。结论:错配修复基因hMSH2IVS12-6(T>C)位点的突变可能为GMA诱导人支气管上皮细胞恶性转化过程中的重要起始分子事件之一。

BACKGROUND AND AIM： To examine the mutation of DNA repair genes of human bronchial epithelial cells malignant transformation induced by glycidyl methacrylate. MATERIALS AND METHODS： To evaluate the mutation of DNA repair genes XRCC1, hMSH2, XPD and XRCC3 by polymerase chain reaction-restriction fragments length polymorphism（PCR-RFLP）, and the result was verified by the DNA sequencing. RESULTS： The mutation of hMSH2 IVS12-6 （T〉 C） was observed, while mutations in the other genes were not found.The results from PCR-RFLP and DNA sequencing were consistent. CONCLUSION：The mutation of DNA repair genes hMSH2 WS12-6 （T〉 C）might be important and an initation step during the malignant transformation of human bronchial epithelial cells induced by glycidyl methacrylate.