TRAIL途径在维生素E琥珀酸酯诱导人胃癌细胞凋亡中的作用

Roles of TRAIL in Vitamine Succinate-induced Apoptosis in Human Gastric Cancer Cells

背景与目的:探讨TRAIL途径在维生素E琥珀酸酯(VES)所诱导的人胃癌MKN28细胞凋亡过程中的作用。材料与方法:人胃癌MKN28细胞,分为VES不同浓度(5、10、20μg/ml)作用的3个实验组,和溶剂对照组(乙醇),共4组。各组受试物作用细胞24h后,采用DAPI染色法观察VES诱导细胞凋亡的情况;采用WesternBlot分析VES各实验组不同作用时间(0、6、12、18、24h)对MKN28细胞的TRAIL相关蛋白(DR4、DR5、DcR1和DcR2)表达的影响。结果:VES明显诱导MKN28细胞凋亡,5、10、20μg/ml实验组VES作用MKN28细胞12h,其DR4和DR5表达增强,而DcR1和DcR2的表达下降;当10μg/ml浓度组VES作用细胞时,随着时间的延长,DR4和DR5的表达逐渐增强,12h达到峰值。结论:TRAIL途径可能参与了VES诱导的MKN28细胞凋亡。

BACKGROUND AND AIM： In order to investigate the roles of TRAIL in vitamin E succinate （VES） -induced apoptosis in human gastric cancer MKN28 cells. MATERIALS AND METHODS： The MKN28 huamn gastric cancer cells were cultured with different concentrations of VES. The MKN28 cells were divided into four groups：5, 10, 20 μg/ml VES and control groups. Apoptosis was assessed by DAPI staining, and TRAIL-receptor（DR4, DR5, DcR1 and DcR2） protein expressions induced by VES at different doses and different time points were measured by western blot. RESULTS： The results showed that VES obviously induced cells to undergo apoptosis.The expression of DR4 and DR5 were increased by VES at 5, 10, 20 μg/ml for 12 h; however the expression of DcR1 and DcR2 were reduced .DR4 and DR5 were increased by the hour, then reached the top level at 12 h. CONCLUSION： The data implicated that TRAIL pathway might be invovled in VES-induced apoptosis.