摘要
采用中性盐沉淀、凝胶层析等常规方法纯化黄粉虫Tenebrio molitor血淋巴中的β-1,3-葡聚糖识别蛋白,并对其在酚氧化酶原激活系统中的作用进行了初步的研究。结果表明:黄粉虫血淋巴的β-1,3-葡聚糖识别蛋白的分子量约为70kDa,主要分布于血浆中。纯化的β-1,3-葡聚糖识别蛋白只能特异性地识别β-1,3-葡聚糖而不能识别肽聚糖。在β-1,3-葡聚糖所诱导的酚氧化酶原的激活过程中,随着酚氧化酶原激活程度的提高,内源性β-1,3-葡聚糖识别蛋白的含量逐渐减少。抗β-1,3-葡聚糖识别蛋白多克隆抗体对黄粉虫血淋巴中由β-1,3-葡聚糖所诱导的酚氧化酶活性起抑制作用,且该抑制作用呈现一种剂量依赖性的趋势。上述结果有助于深入了解β-1,3-葡聚糖对黄粉虫血淋巴酚氧化酶原激活系统的激活作用。
One kind of β-1,3-glucan recognition protein (Tm-βGRP) was purified from Tenebrio molitor larvae by such traditional means as neutral salt precipitation and gel chromatography, etc., and its biological functions in pro-phenoloxidase activation system were primarily studied. The results showed that the molecular weight of Tm-βGRP was approximately 70 kDa, and the protein mainly existed in plasma. Purified Tm-βGRP could only specifically recognize β-1,3-glucan, but not peptidoglycan. During the process of pro-phenoloxidase activation induced by β-1,3-gluean, with the proceeding of prophenoloxidase activation, the amount of endogenous Tm-βGRP decreased significantly. The polyelonal antibody against Tm-βGRP could inhibit phenoloxidase activity in the hemolymph of T. molitor induced by β-1, 3-gluean in a dose-dependent manner. This research may help us deeply elucidate the biological function of β-1, 3-glucan in pro-phenoloxidase activation system in the hemolymph of T. molitor.
出处
《昆虫学报》
CAS
CSCD
北大核心
2009年第1期27-32,共6页
Acta Entomologica Sinica
基金
辽宁省自然科学基金项目(20072066)
关键词
黄粉虫
β-1
3-葡聚糖识别蛋白
分离纯化
生物学功能
酚氧化酶原激活系统
先天性免疫
Tenebrio molitor
β-1, 3-glucan recognition protein
isolation and purification
biological function
pro-phenoloxidase activation system
innate immunity