Smad蛋白在口腔黏膜肌成纤维细胞转分化中的作用

The transdifferentiation mechanism of oral myofibroblasts

目的:探索口腔黏膜成纤维细胞向肌成纤维细胞分化的机制。方法:利用免疫组织化学方法检测成纤维细胞表达α平滑肌肌动蛋白之前表达Smad蛋白的改变。结果:口腔黏膜成纤维细胞(FB)组核阳性率为(17.38±10.36)%;FB与口腔黏膜角质形成细胞(KC)共同培养24hSmad2/3蛋白核表达为(35.33±7.68)%,与FB组相比p=0.015结论:口腔黏膜FB向肌成纤维细胞(MFB)分化的机制与TGFβ-Smad信号途径有关。。

Objective： In order to elucidate the transdifferentiation mechanism of oral myofibroblasts. Method： Detection the expression of Smad protein in fibroblasts before the expression of αsmooth muscle actin. Result： The nuclear positive ratio in group fibroblast was （17.38±10.36）%,while that in group FB＋KC was （ 35.33±7.68）%, significant differention was seen between the two groups.Conclusion： The transdifferentiation mechanisim of oral fibroblast may relate to the TGFβ- Smad signal pathway.