乳牙化学去龋法的细菌学研究

The bacteriological study of primary teeth of chemo-mechnical caries removal

目的:比较化学去龋法和机械去龋法清除细菌的效果。方法:选取40个龋坏深至牙本质的乳磨牙,中龋或深龋,随机分为化学法去龋组和机械法去龋组,每组20个牙,去龋前后使用无菌挖匙对龋坏牙本质进行取样,分别在4种不同的培养基上进行厌氧培养和计数。结果:两种方法去龋后,除机械法去龋组放线菌外,两种方法其他细菌数显著减少,有统计学意义(p<0.05),化学法去龋组比机械法去龋组的总菌数量减少更显著(p<0.05)。结论:化学去龋法比机械去龋法清除乳牙龋坏细菌效果好。

Objective： To compare the efficacy of chemo-mechanical caries removal with conventional excavation in reducing the count of the cariogenic flora. Method： 40 carious deciduous molar teeth were collected and randomly divided into two groups, i.e., conventional drilling group （by bur） and Carisolv group. There were twenty teeth in each group. Samples from carious dentine were taken with a sterile scraping instrument. All softened dentine was removed and after second sample was taken. All samples were serially diluted and cultivated on four different nutrient agar plates, and counted. Result： Both methods produced a statistically significant reduction in bacterial counts after carie s removal,except actinomyces in conventional drilling group. There was significant decrease of the total bacteria in Carisolv group than conventional drilling group （by bur） （P〈0.01）. Conclusion： The Carisolv chemo-mechanical method is relatively more effective than conventional methods.