氧化锆陶瓷和钛合金表面粗糙度与细菌黏附的比较

Comparison of surface roughness and bacterial adhesion on zirconia ceramics and titanium alloys

实验于2006-03/06在山西医科大学微生物实验室完成。分别制作氧化锆陶瓷试件和Titan合金试件各6个。实验分为2组,氧化锆陶瓷抛光组:Cercaon氧化锆薄片磨光后,用EVE专用抛光轮高度抛光。钛合金抛光组:将钛合金原始铸件依次用粗砂纸、水砂纸(依次为200,600,800,1000,1200,1500,2000目)逐级磨光,再用抛光轮抛光。采用精密粗糙度测试仪检测各样本的表面粗糙度;然后将各样本置于变形链球菌的培养液中,在37℃条件下,静止培养状态下培养1h后,荧光显微镜下计数黏附细菌的数量,并通过扫描电子显微镜观察各样本的表面形貌。结果显示,两组间表面粗糙度无显著差异(P>0.05),但钛合金抛光组试件黏附细菌数多于氧化锆陶瓷抛光组试件黏附细菌数(P<0.01)。扫描电镜显示,氧化锆陶瓷抛光组试件表面散在少量细小孔隙,而钛合金抛光组试件表面未见细小孔隙,但有较多裂隙和凹陷。

The experiment was performed at the Microbiology Laboratory of Shanxi Medical University from March to June of 2006. Six specimens of zirconia ceramics and six of titanium alloys were prepared. Specimen in the zirconia ceramics group was polished with EVE buffing wheel. Casting in the titanium alloy group was sequentially polished with buffing wheel and 200, 600, 800, 1 000, 1 200, 1 500, 2 000 grit sanding or waterproof abrasive papers. Initial roughness measurements were obtained by the usage of a roughometer. Then the specimens were separately placed into streptococcus mutans culture fluid and incubated in the condition of same vibrating speed for 1 hour under 37 ℃, after that, the amount of adhered bacteria was counted by fluorescence microscope, surface topography was observed by scanning electron microscope. The results show that there is no significantly difference of roughness values between the two groups （P 〉 0.05）. But the amount of adhered bacteria of surface-polished with titanium alloy is greater than that of surface-polished with zirconia ceramics （P 〈 0.01）. The scanning electron microscope micrographs demonstrate that polishing treatment of titanium alloy has some pores, while numerous leakage and introcession can be found in the surface-polished with zirconia ceramics group.