β-磷酸三钙脱有机质骨的成骨性能

Bone formation performance of beta-tricalcium phosphate sintered bone

背景:人工发孔方法制备的β-磷酸三钙材料在孔隙结构上与天然松质骨的孔隙结构存在巨大的差异,进而影响了其本身的生物学性能。目的:采用体内成骨试验方法验证牛松质骨煅烧制备的β-磷酸三钙脱有机质骨的体内成骨活性。设计、时间及地点:对比观察,动物体内实验,于2005-07/2006-10在解放军总医院骨科研究所完成。材料:将健康牛松质骨脱去有机成分后,经2次高温煅烧方法制备形成β-磷酸三钙脱有机质骨标准试件,经辐照灭菌后备用。方法:健康新西兰大白兔12只,按4,8,12周3个取材时间分成3组,每组4只,将β-磷酸三钙脱有机质骨4个试件随机植入上述3组动物一侧下肢的骨缺损中,同时在每只兔另一侧下肢制备相同骨缺损模型,不植入任何材料,为空白对照。主要观察指标:于材料植入后1d以及4,8,12周分别拍摄兔膝关节正侧位X射线片,观察缺损部位新骨形成情况,并与空白对照组进行对比。结果:随着β-磷酸三钙脱有机质骨植入时间的延长,材料周边及中心部位逐渐有新生骨组织长入,但植入的材料无明显降解吸收,新骨长入量有限。空白对照至12周仅缺损周围有少量新骨生成。结论:β-磷酸三钙脱有机质骨具有体内成骨活性,但降解缓慢影响新骨的替代和改建过程。

BACKGROUND： There is a huge difference between the natural cancellous bone and β -tricalcium phosphate （ β-TCP） materials, which can effect the biology features of the material. OBJECTIVE： To verify the activity of bone formation of β -TCP sintered bone by bone formation. DESIGN, TIME AND SETTING： The in vivo control experiment of animal was performed at the Department of Orthopaedics, General Hospital of Chinese PLA from July 2005 to October 2006. MATERIALS： The test pieces of β -TCP made from cancellous bone in the femur of healthy cattle by sintering were sterilized to prepare to use. METHODS： Twelve New Zealand rabbits were divided into 3 groups according to different sample harvest time, with 4 animals in each group. Cylinder bone defect in the distal femur of rabbits were prepared, and then repaired by test pieces of β -tricalcium phosphate, the contrast side was served as the blank control. MAIN OUTCOME MEASURES： The condition of the defect repairing was observed by X-ray image, additionally, to compare with the blank control group at 1 day, 4, 8, and 12 weeks after implantation. RESULTS： Follow the time prolonged, there were new bones grow into the edge and center of the materials gradually, but the materials had no obvious degradation or absorption, the quantity of new bone was limited. At 12 weeks, only a few of new bones could be found in the edge of defect area of the blank control group. CONCLUSION： β -TCP sintered bone has the ability of bone formation in vivo, but its slowly degradation hinder the process of new bone substituting and rebuilding.