利用DNS定糖法快速测定烟草中水溶性总糖的研究

Study on Rapid Mesuring Soluble Sugar by DNS Method

研究了ＤＮＳ定糖法应用于烟草中水溶性糖的测定，探讨了萃取剂、脱色与除蛋白方法、ＨＣｌ水解时间等因素对ＤＮＳ法测糖的影响。研究结果表明：该方法与Ｂｅｒｔｒａｎｄ方法相比具有较高的准确度，且与邻甲苯胺法相比具有较好的显色稳定性。该法应用于烟草，一次可以测定几十个样品，用此方法与流动注射分析技术（ＦＩＡ）相结合，可实现批量测定的操作自动化。

DNS sugar determination method was established for soluble total sugar in tobacco .Compared with stadard method in tobacco industry-Bertrand method,DNS method is simpler,more rapid,more accurate and suitable for sugar mesurement in tobacco and has relative error within 5% and accuracy of 99.5% .An extraction procedure for sugar in tobacco was established,20% alcohole solution as extractent and active carbon as decolorizer.