一株产槲皮素类色素的银杏内生真菌的研究(英文)

An Endophytic Fungus Producing Orange Pigment Isolated from Ginkgo Biloba L.

从银杏树根中分离出70余株内生真菌。将它们置于马铃薯葡萄糖(PD)液体培养基中培养,发现其中一株(Gh01)能产生橙黄色色素。经过化学反应及HPLC检测证明该橙黄色色素为槲皮素类糖苷。这是关于内生真菌产槲皮素类糖苷的首次报道。本文深入探讨了碳源,氮源,金属离子,初始PH及培养温度对色素产量的影响。PD液体培养基的最适培养温度和初始PH分别为28℃和7.0。正交设计结果显示:最适碳源和氮源分别为20g/L葡萄糖和5g/L蛋白胨。增加1g/L的氯化锌可提高色素产量。在最适培养条件下连续培养120h色素的产量可达到27.515g/L。

More than seventy endophytic fungi were isolated by aseptic techniques from the phloem of the root of Ginkgo biloba L.. They were cultured in the potato dextrose （PD） liquid medium and one endophytic fungus （Gh01） was screened out having the capacity of producing orange pigment. The orange pigment was identified as quercetin glycoside by chemical reaction and HPLC. This is the first report on quercetin glycoside produced by endophytic fungus. The effects of the carbon and nitrogen sources, metal ions, initial pH and incubation temperature on pigment production of the endophytic fungus were evaluated. The optimal temperature and initial pH for pigment production in PD liquid medium were 28 ℃ and 7.0, respectively. The result of orthogonal trial showed that the most suitable carbon and nitrogen source were 20 g/L glucose and 5 g/L peptone respectively; 1 g/L chloridize zinc could increase the yield of pigment. The maximum yield of the pigment was 27.515 g/L after 120 hours＇ successive culture under the optimal conditions established.