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Synaptotagmin I和IV在LβT2细胞中的定位和shRNA基因干扰研究 被引量：2

The Localization of Synaptotagmin I,IV and the Effect of shRNA in LβT2 Cells
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摘要 目的:研究Sytnaptotagmin(Syt)I和IV在LβT2细胞中的定位和shRNA干扰技术对这两种蛋白在促性腺激素释放细胞(LβT2)细胞中蛋白表达量的影响。方法:运用全内反射荧光显微系统,通过在LβT2细胞中共表达各种细胞器的蛋白标记物和SytI、IV与荧光蛋白的融合蛋白,对SytI、IV进行细胞内定位。并运用shRNA干扰技术对SytI、IV在LβT2细胞中蛋白表达量进行了干扰。结果:发现了SytI、SytIV在LβT2细胞中和致密核心大囊泡(LDCV)都存在了一定的共存比例,而且shRNA技术对SytI和SytIV在LβT2细胞中的表达沉默产生了一定的效用。结论:SytI、SytIV在LβT2细胞中与致密核心大囊泡共存比例很大,shRNA干扰技术对SytI、SytIV的沉默具有高效性,为未来进一步对SytI、SytIV在LβT2细胞中的功能研究打下了基础。 Objective： To observe localizations of Syt I, and Syt IV in LβT2 cells and test the expression of Syt I, IV in LβT2 cells after silenced by shRNA. Methods： We used the total internal reflection fluorescence microscopy（TIRFM） to observe localizations of Syt I, and Syt IV in LβT2 cells, and silenced the expression Syt I,IV by shRNA technology. Results： The fusion proteins of Syt I, IV with fluorescent proteins are mostly co-localized with the maker of the large dense vesicle. And shRNA technology has a good efficiency on silencing Syt I, Syt IV in LβT2 cells. Conclusions： Syt I, Syt IV are colocalized with LDCV in LβT2 cells. The technology of shRNA for Syt I and Syt IV has a good efficiency and it is useful for the Syts function research in LβT2 cells.
作者 杨帆 赵平
机构地区 华中科技大学生命科学与技术学院
出处 《现代生物医学进展》 CAS 2009年第2期355-357,共3页 Progress in Modern Biomedicine
基金 国家重点基础研究发展计划资助项目(2004CB720000 2006CB0D1704)
关键词 SYNAPTOTAGMIN RNA干扰 LβT2细胞 全内反射荧光显微镜 Synaptotagrnin RNAi LβT2 cell Total internal reflection fluorescence microscopy
分类号 Q503 [生物学—生物化学] Q813 [生物学—生物工程]
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同被引文献32

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现代生物医学进展
2009年 第2期

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