青海双峰驼血液LDH和SOD同工酶酶谱

Isozyme-spectra of Lactate Dehydrogenase and Superoxide Dismutase of Blood in Qinghai Camelus Bactrianus

采用聚丙烯酰胺凝胶电泳法对５８峰青海双峰驼的红细胞和血清乳酸脱氢酶（ＬＤＨ）以及红细胞超氧化物岐化酶（ＳＯＤ）同工酶酶谱进行了分析和研究。结果：①Ｓ－ＬＤＨ同工酶酶谱存在年龄差异，成年驼只有ＬＤＨ１同工酶，幼年驼的酶谱由ＬＤＨ１、ＬＤＨ２、ＬＤＨ３和ＬＤＨ４组成；②ＲＢＣ－ＬＤＨ同工酶由ＬＤＨ１、ＬＤＨ２和ＬＤＨ３组成，其活性强于Ｓ－ＬＤＨ；③ＳＯＤ呈现单一的同工酶酶谱，由ＳＯＤ１、ＳＯＤ２和ＳＯＤ３组成；④ＳＯＤ１同工酶的相对迁移率最大（８０．３％），ＬＤＨ４的最慢（２１．４％）。

58 Qinghai camelus bactrianus were used to analyse and investigate the isozymespectra of lactate dehydrogenase (LDH) and superoxide dismutase(SOD) in blood by means of polyacrylamide gel electrophoresis. The results were as follows:(1) There was a difference between the camelus bactrianus at variant agegroups on the SLDH isozymespectra: the pattern contained only single LDH1 in adult group and the pattern consisted of LDH1, LDH2, LDH3 and LDH4 in young group. (2) The RBCLDH isozymes consisted of LDH1, LDH2 and LDH3, and its enzyme activity was stronger than that of SLDH. (3) There was single SOD isozymespectrum consisted of SOD1, SOD2 and SOD3. (4) The relative moving rate of SOD1 was the fastest (80.3%) and that of LDH4 was the slowest (21.4%).