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零价铁对铬污染底泥的修复及其对微生物群落结构的影响 被引量:6

Remediation of chromium-contaminated sediments by zero-valent iron and its effects on microbial community structure
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摘要 采用实验室批次实验方法,研究了零价铁对Cr(Ⅵ)模拟污染底泥的修复作用.结果表明,添加2%(以底泥干重计)的还原铁粉在24h内可将150mg·kg-1的Cr(Ⅵ)去除94.7%,较未加还原铁粉的Cr处理提高23.7%,较S+Cr+Fe处理(灭菌加铁)高14.2%;这说明,零价铁修复有效地促进了底泥中Cr(Ⅵ)的去除,底泥中的土著微生物也具有一定的去除Cr(Ⅵ)的作用.采用变性梯度凝胶电泳(Denatured Gradient Gel Electrophoresis,DGGE)和磷脂脂肪酸(Phospholipid Fatty Acid,PLFA)分析技术研究了零价铁修复对底泥微生物群落结构的影响.基于DGGE图谱的差异条带DNA序列分析和多样性指数分析表明:零价铁修复可使底泥中Cr(Ⅵ)耐受菌群减少,敏感菌群重新出现,并且可使底泥微生物的Shannon多样性指数恢复至未污染对照水平.PLFA指纹图谱分析结果表明:零价铁修复可使代表细菌生物量的磷脂脂肪酸17∶0的量以及Cr(Ⅵ)对底泥微生物的胁迫恢复至未污染对照水平. Laboratory batch experiments were carried out to determine the effectiveness of zero-valent iron (ZVI) on the rcmediation of Cr( Ⅵ )- contaminated sediments. This study showed that the removal rate of Cr(Ⅵ) was 94.7% with ZVI in 24 hours. This was 23.7% higher than the treatment without ZVI, and 14.2% higher than treatment with both sterilization and ZVL These results indicated that ZVI alone can efficiently remove Cr( Ⅵ ), and the indigenous microorganisms in sediments also can contribute to the removal. Microbial community structures were monitored by 16S rDNA PCR- Denaturing Gradient Gel Electrophoresis ( DGGE ) and Phospholipids Fatty Acid (PLFA.) analysis to identify the microbial community shift after the remediation of Cr( Ⅵ ) using ZVI. DGGE analysis showed that species sensitive to Cr( Ⅵ ) increased after remediation by ZVI, and species resistant to Cr (Ⅵ) decreased. Shannon' s index of diversity of the sediment microbial community, which was calculated from 16S rDNA PCR-DGGE data, was also restored to the control level. The results of PLFA analysis demonstrated that the content of the 17:0, as the biomarker associated with bacteria biomass, and the Cr( Ⅵ ) stress on the microbes were both restored to the control level by using ZVI.
出处 《环境科学学报》 CAS CSCD 北大核心 2009年第2期297-304,共8页 Acta Scientiae Circumstantiae
基金 国家重点基础研究计划(973)项目(No.2004CB418505) 国家自然科学基金资助项目(No.30670391)~~
关键词 零价铁 六价铬 底泥 微生物群落结构 变性梯度凝胶电泳 磷脂脂肪酸 zero-valent iron (ZVI) Cr( Ⅵ ) sediments microbial community structure DGGE PLFA
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