褪黑素通过提高p53和Fas的表达促进小鼠肝癌H22细胞凋亡

Melatonin Induced Apoptosis of H22 Cells by Increasing Expression of p53 and fas

目的:研究褪黑素(MLT)对小鼠肝癌细胞株H22的促凋亡作用及其机理。方法:采用丫啶橙(AO)染色、培养液乳酸脱氢酶(LDH)活性检测和流式细胞术(FCM)观察MLT的促凋亡作用;采用RT-PCR方法检测MLT处理前后细胞的p53mRNA、FasmRNA的水平。结果:AO染色后H22细胞呈现明显核浓缩的凋亡形态;培养液LDH活性检测及FCM分析均提示MLT诱导H22细胞发生凋亡;RT-PCR结果显示p53、Fas表达增强。结论:MLT能促进H22细胞p53和Fas的表达,从而诱导细胞发生凋亡。

Objective： To investigate the mechanism of apoptosis effect on H22 hepatoma cells induced by melatonin （MLT）. Methods： Apoptosis effect of MLT on H22 cells was studied by acridine orange（AO） staining, lactate dehydrogenase（LDH） assay and flow cytometry（FCM）. Comparing the levels of p53 mRNA and Fas mRNA between cells treated with and without MLT by RT-PCR. Results： The nuclear shrinking and fragments were obvious after AO staining. LDH activity increased after MLT treatment, and a higher rate of apoptosis was observed by FCM. The expressions of p53 and Fas gene was stimulated by MLT（P〈0.01）. Conclusion： MLT induced apotosis of the H22 cells dependent on p53 and Fas.