欧洲甜樱桃幼胚子叶离体培养再生植株研究

Adventitious Shoot Regeneration in Vitro from Immature Cotyledon of the Sweet Cherry

以欧洲甜樱桃（Prunus avium L.）幼胚子叶为试材，研究不同品种、不同子叶发育时期、6-BA和NAA配比和培养条件等对其离体再生不定芽的影响。结果表明，以MS为基本培养基，附加6-BA 2.0mg·L^-1＋NAA 0.2mg·L^-1，用PF（子叶的长度／胚的长度×100）=50-80发育阶段的子叶，‘那翁’品种幼胚子叶再生不定芽效果最好，再生率最高可达79.2％。暗培养2周后再转入光照下培养，对子叶再生不定芽具有一定的促进作用。‘雷尼尔’品种子叶再生率为72.2％，‘先锋’为61.1％，‘拉宾斯’为33.3％。子叶再生不定芽全部发生于子叶正面近胚芽端切口处，表现出明显的极性效应。

The aim of this experiment was to examine the effects of the different development stage of cotyledon, 6-BA and NAA concentration, cuhivar and culture condition on adventitious shoot regeneration from the immature cotyledon explants of the sweet cherry （Prunus avium L. ）. The results showed that MS culture medium with 6-BA 2.0 mg·L^-1＋ NAA 0.2 mg·L^-1 was the optimal for the adventitious shoot regeneration in the euhivar ‘Napoleon＇ and the optimal size of the immature cotyledon was PF （ cotyledon length/embryo length×100）=50-80 and the regeneration rate reached to 79.2%. Dark culture for 2 weeks prior to light culture was helpful to adventitious shoot regeneration. Shoot regeneration rate also reached to 72.2% in ‘Rainier＇ , 61.1% in ‘Van＇ and 33.3% in ‘Lapins＇. Adventitious shoots were mainly regenerated at prox- imal cutting region of cotyledon adaxial surface.