摘要
目的:建立荧光定量聚合酶链反应(FQ-PCR)法检测CK19基因表达水平,探讨其在乳腺癌诊断和治疗监测中的应用。方法:建立FQ-PCR法,并以β2-微球蛋白为内对照测定30名健康女性体检者、30例良性乳腺疾病患者和91例乳腺癌患者外周血中CK19的表达。结果:CK19表达水平在正常对照组和良性乳腺疾病组间差异无显著性意义(P>0.05),乳腺癌组均高于前两组(P<0.05),β2-微球蛋白在三组间差异无显著性意义(P>0.05)。91例乳腺癌患者阳性率为49.5%,良性乳腺疾病组为0。CK19表达与患者年龄和肿瘤大小和类型无关(P>0.05),与乳腺癌临床分期、组织学分级以及腋淋巴结转移相关(P<0.05)。结论:FQ-PCR技术是高度灵敏、高度特异的快速定量检测CK19方法,可有效监测乳腺癌的诊断、疗效、转移和预后。
Objective:To evaluate the clinical application of fluorescent quantification polymerase chain reaction (FQ- PCR) to detect CK19 in the breast cancer. Methods: CK19 in 30 health women, 30 patients with benign breast disease and 91 patients with breast cancer were detected by FQ - PCR. β2 - microglobin was used as internal control. Results: There were no significant difference in CK19/β2 -microglobin between normal controls and benign breast disease group(P 〉 0.05 ). CK19/β2- microglobin in breast cancer group were higher than those in the other groups( P 〈 0.05 ). There were no difference in β2 -microglobin among three groups( P 〉 0.05 ). The positive ratio of breast cancer and benign disease were 49.5 and 0 percent respectively. No significant association of CK19 expression among age and tumor size and type was noted ( P 〉 0.05 ) , but its expression in breast cancer was correlated with clinical stage, histological grade and axillary metastatic lymph node (P 〈 0.05 ). Conclusion: FQ -PCR is a rapid and sensitive and specific method for quantitating CK19. It also gives objective evidence to the diagnosis, therapy, metastasis of breast cancer.
出处
《现代肿瘤医学》
CAS
2009年第2期243-245,共3页
Journal of Modern Oncology
基金
四川省青年科技基金资助项目(编号:2007-05-345)