膀胱移行细胞癌中肿瘤-睾丸抗原基因的表达情况

Expression profile of cancer-testis genes in urinary transitional cell carcinomas

目的:研究4种肿瘤-睾丸抗原(CT)基因在膀胱移行细胞癌中的表达及其临床意义。方法:采用反转录聚合酶链反应(RT-PCR)技术检测49例膀胱移行细胞癌患者癌组织(新鲜标本,Ta-T1期28例,T2-T4期21例;G120例,G216例,G313例)及其中15例患者癌旁组织的cTAGE-1、cTAGE-2、MAGE-A1及NY-ESO-1等4种CT基因mRNA的表达。结果:49例膀胱移行细胞癌组织中MAGE-A1表达最高,其次为cTAGE-1,cTAGE-2及NY-ESO-1,分别为59%(29/49),55%(27/49),51%(25/49)及47%(23/49)。15例癌旁组织表达均阴性。膀胱移行细胞癌组织中肿瘤不同分期、不同分级之间4种CT基因表达的差异均无统计学意义(Pearsonχ2检验法,P>0.05)。结论:CT基因在膀胱移行细胞癌组织中有较高表达,而在癌旁组织无表达,可以进一步研究作为膀胱移行细胞癌特异性免疫治疗靶基因的可行性。

Objective：To evaluate the significance of 4 individual cancer- tesits （CT） gene expression in bladder transitional cell carcinomas. Methods ： cTAGE - 1, cTAGE - 2, MACE - A1, NY - ESO - 1 expression were detected by reverse transcription polymerase chain reaction （RT- PCR） in 49 samples of bladder transitional cell carcinoma patients （Ta -T1 28 samples ,T2 -T4 21 samples, G1 20 samples, G2 16 samples, G3 13 samples） and 15 samples of adjacent tissues. Results The CT gene was detected with various frequencies in bladder transitional cell carcinoma tissue： MAGE - A1 ,59% （29/49） ; cTAGE - 1,55% （ 27/49 ） ; cTAGE - 2,51% （25/49） and NY - ESO - 1,47% （23/49）. In contrast, no CT gene expression was found in cancer adjacent tissues. We did not find any significant correlation between CT gene expression and disease stage or grade （P 〉 0.05 ）. Condusion： CT gene highly expressed in bladder transitional cell carcinoma and no CT gene expression was found in cancer adjacent tissues. It＇s probability to be target gene in bladder transitional cell carcinoma immunotherapy should be further explored.