摘要
本研究对大肠杆菌表达的重组牛白细胞介素－２的生物活性单位进行了测定。以已标定的人白细胞介素－２为标准品，ＭＴＴ比色法测出重组牛白细胞介素－２的生物活性单位约为４×１０６ｕ／Ｌ菌液。同时，以ＳＤＳ－ＰＡＧＥ法测出表达的重组牛白细胞介素－２分子量约为１５．５ＫＤ。理化性质分析结果表明，重组牛白细胞介素－２生物活性酸碱适应范围约为２～１０；对胰蛋白酶和木瓜蛋白酶的作用敏感，而对核酸酶的作用则不敏感；重组牛白细胞介素－２对温度变化敏感，表现为不耐高温。
In this study we measured the biological activities of recombinant bovine interleukin 2(rBoIL 2)produced by E.coli.The specific activity of the rBoIL 2 was about 4×10 6u/L of bacterium suspension in MTT colorimetric assay.The rBoIL 2,with the apparent molecular weight of 15 5KDa,was stable at pH2 10 and resisted DNase and RNase treatments,but was very sensitive to papain and pronase.The activity of rBoIL 2 depressed rapidly when the temperature increased over 37℃.
关键词
重组牛
白细胞介素-2
生物活性
理化性质
Recombinant bovine interleukin 2(rBoIL 2) MTT colorimetric assay
