摘要
１日龄非免疫鸡，ＨＶＴ常规剂量免疫后，于第３周起，每周采集一次羽毛根，分别作常规ＭＤＶ琼扩抗原的检测，结果显示：在免疫后第３～８周检测，均未观察到特异性ＡＧＰ沉淀线；若取较多数量的富含羽髓的羽毛根，经２ｍｌ磷酸缓冲液（ＰＢＳ）浸提、浓缩胶浓缩约４０倍后，再作上述检测，则分别于免疫接种后的第３～５周都有特异性的ＡＧＰ沉淀线出现，且与感染细胞上清中浓缩的ＨＶＴ及ＭＤＶ琼扩抗原所形成的沉淀线基本吻合，而第６周检测时，仅与ＨＶＴ阳性血清呈微弱阳性，用同样方法对免疫后第６周后的检测结果均表明为阴性。由此推断，免疫鸡羽毛根中含有较低水平的、并与致病性ＭＤＶ抗原具有交叉反应性的ＨＶＴ琼扩抗原，但采用常规ＡＧＰ法不能直接检测到。
One day old chickens without maternal antibody were vaccinated with the commercial turkey herpesvirus (HVT) Starting on weed 3 and about 1 week intervals,the feather tips or pulp liquid of the birds were detected by agar gel precipitiation test (AGP) for the viral antigens directly.Results showed it was always negative at 3 to 8 weeks post vaccination (PV) However,when 20 to 40 feather tips were pooled and cut into pieces in 2ml phosphate buffered saline (PBS),the suspension after incubation was centrifuged and the buffer decreased by concentrated gel to about 1/40 times in volume was detected by AGP for viral antigens It showed that the viral antigens in the feather was recovered and always positive for both HVT and Marek’s disease virus (MDV) antigens at 3 to 5 weeks PV,whereas it was only positive for HVT antigens at 6 weeks and none at 7 to 8 weeks PV Results were deduced that the viral antigens for HVT could be found at low levels in feather tips of chickens vaccinated with HVT,but couldn’t be recovered directly by the conventional AGP.
