异源猪瘟病毒C株E2基因保护性抗原编码区的序列分析与比较

Sequence Analysis and Comparison of Protective Antigen Encoding Region of E2 Gene of Hog Cholera Virus Strain C from Different Sources

应用ＲＴ－ＰＣＲ扩增了我国南京、成都、吉林３个兽医生物制品厂提供的猪瘟病毒兔化弱毒株（Ｃ株）的Ｅ２基因保护性抗原编码区，然后将其克隆到ｐＧＥＭ－Ｔ载体中，用Ｓａｎｇｅｒ′ｓ双脱氧法对重组质粒中的插入片段进行了序列分析。将测得的这３个厂生产的Ｃ株的核苷酸序列及其推导的氨基酸序列与作者实验室测得的国内石门株和国外测得的Ｃ株相同区域进行同源性比较，发现不同来源的Ｃ株及石门株之间存在不同程度的差异，核苷酸同源性为９３．６％～９９．６％，氨基酸同源性为９０．６％～９８．９％。

The major protective antigen encoding regions of E2 gene of hog cholera virus (HCV) strain C from 3 veterinary biological product factories were amplified by RT PCR. Then they were cloned and sequenced by Sanger′s method. Comparison and analysis of the necleotide sequences and deduced amino sequences among the strains C from different sources was performed by computer with DNASIS software. The results showed some substitutions of nucleotide or deduced amino acid scattered along E2 gene or its encoding protein with the homology of the nucleotide and amino acid sequences being 93.6%￣99.6% and 90.6%￣98.9%, indicating HCV strain C would have varied genetically to a certain extent in the course of many years′ mutiplication and amplification.