摘要
应用RT-PCR扩增了我国南京、成都、吉林3个兽医生物制品厂提供的猪瘟病毒兔化弱毒株(C株)的E2基因保护性抗原编码区,然后将其克隆到pGEM-T载体中,用Sanger′s双脱氧法对重组质粒中的插入片段进行了序列分析。将测得的这3个厂生产的C株的核苷酸序列及其推导的氨基酸序列与作者实验室测得的国内石门株和国外测得的C株相同区域进行同源性比较,发现不同来源的C株及石门株之间存在不同程度的差异,核苷酸同源性为93.6%~99.6%,氨基酸同源性为90.6%~98.9%。
The major protective antigen encoding regions of E2 gene of hog cholera virus (HCV) strain C from 3 veterinary biological product factories were amplified by RT PCR. Then they were cloned and sequenced by Sanger′s method. Comparison and analysis of the necleotide sequences and deduced amino sequences among the strains C from different sources was performed by computer with DNASIS software. The results showed some substitutions of nucleotide or deduced amino acid scattered along E2 gene or its encoding protein with the homology of the nucleotide and amino acid sequences being 93.6% ̄99.6% and 90.6% ̄98.9%, indicating HCV strain C would have varied genetically to a certain extent in the course of many years′ mutiplication and amplification.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1998年第2期112-114,共3页
Chinese Journal of Veterinary Science
基金
国家自然科学基金
关键词
猪瘟病毒
免化弱毒株
E2基因
序列分析
hog cholera virus
C strain
E2 gene
sequence analysis and comparison