摘要
根据猪繁殖-呼吸道综合征病毒(PRRSV)美洲型毒株和欧洲型毒株的基因组序列设计合成了2对引物,即1008PS/1009PR和1010PLS/1011PLR。我国分离的CH-1a株用这2对引物均能扩增出与预期大小相符的RT-PCR产物,分别与美洲型代表株(VR-2332)的RT-PCR产物大小相当。特异性试验表明,这2对引物均不能扩增其他常见的繁殖障碍相关病毒的RNA或DNA以及未感染的MARC-145细胞RNA。间接免疫荧光试验也证明,CH-1a株与PRRSV核衣壳蛋白特异性单克隆抗体以及美洲型毒株抗血清均呈阳性反应。因此初步证实CH-1a株为美洲型PRRSV。
CH 1a isolated from an outbreak of PRRS in a pig farm in China. Two sets of oligonucleotide primers, 1008PS/1009PR and 1010PLS/1011PLR were designed according to the sequences of PRRSV strain IAF exp91 and LV (European prototype) to determine the genotype of the PRRSV strain. The results showed that PRRSV strain CH 1a could be amplified using either primer pair by RT PCR, yielding a single band of the predicted size after agarose gel electrophoresis, to be similar in size to the products of strain VR 2332. No amplification was observed when RNAs were extracted from hog cholera virus (HCV) and uninfected MARC 145 cells or DNAs from pseudorabies virus (PrV). MARC 145 cells infected with strain CH 1a were shown to react with antisera against strains VR 2332 and NVSL (American isolate) by indirect immunofluorescence staining. Thus, it is concluded that the Chinese PRRSV strain CH 1a belongs to American genotype.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1998年第2期118-121,共4页
Chinese Journal of Veterinary Science