不同品种肉鸡肠道rBAT和y^＋LAT2 mRNA表达的发育性变化

Ontogenetic Expression of rBAT and y^+LAT2 mRNA in Intestine of Broiler

选用遗传背景相同的1日龄父母代雄性Arbor Acre(AA)和父母代雄性岭南黄肉雏鸡各160羽,采用相对定量RT-PCR方法,以AA肉鸡和岭南黄肉鸡十二指肠、空肠和回肠样品为模板,研究不同品种肉鸡肠道碱性氨基酸转运载体rBAT和y+LAT2 mRNA表达的发育性变化。结果显示:十二指肠和空肠rBAT和y+LAT2 mR-NA表达的发育性变化与回肠有着较大的差异。不同品种肉鸡rBAT和y+LAT2 mRNA在十二指肠和空肠的表达具有相同的发育模式,从2~30 d不断升高,44 d下降,55 d回升;岭南黄肉鸡rBAT和y+LAT2 mRNA表达丰度的变化幅度小于AA肉鸡,AA肉鸡rBAT和y+LAT2 mRNA表达丰度在2~30 d时均高于岭南黄肉鸡。回肠rBAT和y+LAT2 mRNA表达的发育性变化在岭南黄肉鸡和AA肉鸡间有显著差别,岭南黄肉鸡回肠rBAT和y+LAT2 mRNA表达在后期(30~58 d)高于前期(2~16 d);而AA肉鸡回肠rBAT和y+LAT2 mRNA表达在58d显著高于其它时间点,在16 d时最低。以上结果说明:(1)十二指肠和空肠rBAT、y+LAT2 mRNA表达的发育性变化与回肠有着较大的差异,这表明肠道近端和远端在碱性氨基酸吸收的功能上可能有差异;(2)不同品种肉鸡十二指肠和空肠rBAT、y+LAT2 mRNA的表达具有相同的发育模式,但在时间点上有一定的差异,而与回肠的表达模式不同,表明rBAT和y+LAT2 mRNA的表达受到发育阶段、品种和肠段的影响;(3)回肠rBAT和y+LAT2mRNA表达的发育性变化在岭南黄肉鸡和AA肉鸡间有显著差别。

160 1-day-old parental male Arbor Acre （AA） broilers and 160 1-day-old parental male Lingnan Yellow broilers were randomly divided into 4 replicates, respectively. Ontogenetic expression of rBAT and y^＋ LAT2 mRNA in duodenum, jejunum and ileum of AA broilers and Lingnan Yellow broilers were determined by relative quantitative RT-PCR. It was found that： （1） Ontogenetic expression of rBAT and y^＋ LAT2 mRNA in duodenum and jejunum was different from that in ileum. （2） Ontogeny patterns of rBAT and y^＋ LAT2 mRNA in duodenum and jejunum were consistent between AA broilers and Lingnan Yellow broilers. The abundance of rBAT and y^＋ LAT2 mRNA in two breeds was increased from day 2 to day 30, then decreased on day 44 and increased again on day 58. While compared two breeds directly, the change range of rBAT and y^＋ LAT2 mRNA in AA broilers was larger than that in Lingnan Yellow broilers. The abundance of rBAT and y^＋ LAT2 mRNA in AA broilers was higher than that in Lingnan Yellow broilers from day 2 to day 30. （3） Ontogeny patterns of rBAT and y^＋LAT2 in ileum was different between two breeds. These results indicate that ： （1） Ontogeny patterns of rBAT and y^＋ LAT2 mRNA in duodenum and jejunum was different from that in ileum, indicating that the function of cationic amino acid absorption in proximal intestine may be different from that in distal intestine. （2） Ontogeny of rBAT and y^＋ LAT2 mRNA in two breeds had same pattern in duodenum and jejunum, and there was difference at same development stage between AA broilers and Lingnan Yellow broilers and ontogeny pattern between duodenum （jejunum） and ileum indicating that rBAT and y^＋ LAT2 mRNA expression could be regulated by developmental stage, breed and segment of intestine. （3） Ontogeny of rBAT and y^＋ LAT2 mRNA in ileum were different between Lingnan Yellow broilers and AA broilers.