短葶山麦冬药材定性与定量分析方法研究

Studies on the quality and quantity analysis methods of Liriope muscari(Decne.)Baily

目的:建立短葶山麦冬药材主要药用成分的定性与定量分析方法。方法:采用薄层色谱法对短葶山麦冬药材中甾体皂苷类成分进行定性鉴别;采用紫外-可见分光光度法对短葶山麦冬药材中多糖成分进行含量测定,以苯酚-硫酸显色,在490nm的波长处测定吸收度。结果:应用所建立的方法对14批药材进行定性与定量分析,结果在TLC图谱中均可检出短葶山麦冬药材的特征斑点;14批样品多糖含量测定结果以无水葡萄糖计为6.34%～13.69%。结论:本方法操作简便,重复性好,结果准确可靠,可用于该药材的定性与定量分析。

Objective ：To establish the quality and quantity analysis methods of Liriope muscari （ Decne. ） Baily. Method：With TLC to identify steroid glycoside of Liriope muscari （Decne.）Baily;Uhraviolet spectrophotometry and colourimetry was used to determinate polysaccharide contents of Liriope muscari （Decne.） Baily, and the detected wavelength was 490 nm. Result： 14 samples were determinated by the new method. The characteristic spots of Liriope muscari（ Decne. ） Baily could be easily examined by TLC. The polysaccharide contents of Liriope muscari （ Decne. ）Baily were 6.34% - 13.69%. Conclusion：These methods are simple, accurate and can be used for the quality and quantity analysis of Liriope muscari （Decne.）Baily.