西洋参根及茎叶皂苷提取物中12种主要皂苷成分的分析研究

RP-HPLC determination of twelve ginsenosides in extract of root,and stem and leaf from Panax quinquefolius L.

目的:测定西洋参根及茎叶皂苷提取物中12种单体人参皂苷的含量。方法:采用WatersAlliance2690液相色谱仪,配Waters996型-PDA检测器,XTerraRP18色谱柱(3.9mm×150mm,5μm),乙腈(A)-水(B)为流动相,梯度洗脱[0min(20%A)→20min(20%A)→25min(25%A)→45min(26%A)→55min(27%A)→70min(32%A)→80min(47%A)→85min(53%A)→90min(53%A)],流速为1.0mL.min-1,检测波长203nm,柱温40℃。结果:12种人参皂苷Rg1、Rg2、Rg3、Re、Rd、Rh1、Rh2、Rf、Rc、Rb1、Rb2和Rb3浓度在其各自的线性范围内,与峰面积呈良好的线性关系,r值均>0.99(n=6);回收率在91.0%～100.4%范围内。结论:本方法精密度和重现性良好,适用于西洋参根及茎叶皂苷提取物中的单体人参皂苷的分析研究,可用于控制西洋参根及茎叶皂苷提取物的质量。

Objective ： To establish an RP - HPLC method for determination of the contents of twelve active constituents ginsenoside Rg1, Rg2, Rg3, Re, Rd, Rh1 , Rh2, Rf, Rc, Rb1 , Rb2 and Rba,in extract of root, and stem and leaf from Panax quinquefolius. Method：The separating was performed on a XTerra RP18 column （3.9 mm × 150 mm,5 μm）. The mobile phase was acetonitrile （A） - water （ B ）, with gradient elution [ 0 min （ 20% A） →20 min （ 20% A）→25 min（25% A）→45 min（26% A）→55 min（27% A）→70 min（32% A）→80 min（47% A）→85 min （53% A）→90 min（53% A） ] at a flow -rate of 1.0 mL · min^-1. Detection was at 203 nm,and the column temperature was set up at 40℃. Results： Rg1 , Rg2, Rg3, Re, Rd, Rh1 , Rh2, Rf, Rc, Rb1 , Rb2 and Rbahad good linear relation in their linear ranges （ r ≥ 0. 99 ） , the average recoveries were between 91.0% - 100. 4%. Conclusion： This method is accurate and reproducible as well as specific and can assess the quality of extract of root, and stem and leaf from Panax quinquefolius.