毛细管气相色谱法测定伤湿祛痛膏中樟脑和薄荷脑的含量

Capillary gas chromatography determination of camphor and menthol in Shangshiqutong plaster

目的:建立毛细管气相色谱法同时测定伤湿祛痛膏中樟脑和薄荷脑的含量。方法:以正十五烷为内标物,采用DB-WAX弹性石英毛细管柱(30mm×0.25mm,0.25μm),FID检测器;氮气为载气;柱温为130℃,检测器温度为200℃;分流比为12:1。结果:在本文色谱条件下,樟脑和薄荷脑及内标物正十五烷均得到良好的分离;樟脑和薄荷脑的加样回收率(n=6)分别为100.1%和99.72%;用所建立的方法对18批样品进行含量测定,均取得满意的结果。结论:本法灵敏、准确,重复性好,可用于该制剂的质量控制。

Objective：To establish a capillary gas chromatography（CGC） method for the determination of camphor and menthol in Shangshiqutong piaster. Method： The internal standard substance of n - pentadecane was employed. The GC system consisted of a DB - WAX capillary column （30 mm × 0. 25 mm, 0. 25 μm）, nitrogen as the cartier gas,column temperature at 130 ℃ ,FID as the detector at 200 ℃ ,and with a split ratio of 12： 1. Result：The contents of camphor and menthol in 18 lots of samples were determined and the constituents concerned have been separated well under the chromatographic condition. The average recoveries （ n = 6） of camphor and menthol were 100. 1% and 99.72% , respectively. Conclusion：The method is simple, accurate, specific and suitable for the quality control of the preparation.