新疆褐牛和中国荷斯坦牛POU1F1基因第六外显子多态性与产奶量的关联分析

Correlations Between POU1F1 Polymorphism and Milk Yield in Xinjiang Brown Cattle and Holstein

以新疆褐牛和中国荷斯坦牛为研究对象,应用PCR-RFLP方法对POU1F1基因第6外显子的多态性及其与产奶量的相关性进行了分析。结果表明,新疆褐牛和中国荷斯坦牛的群体在该位点分别有2种等位基因(A/B),频率分别为0.43/0.57和0.33/0.67,B等位基因是优势等位基因。在新疆褐牛群体中,BB型个体的产奶量显著高于AA型(P<0.05)。

PCR-RFLP method was conducted to detect single nucleotide polymorphism of exon 6 of POU1F1 in Xinjiang Brown Cattle and Holstein. The results showed that both alleles （A/B） were found in Xinjiang Brown Cattle and Holstein, allele B was predominant. Milk yield of the individuals with genotype BB were higher than AA in Xinjiang Brown Cattle（P〈0.05）.