甘肃马鹿血液蛋白多态性及其与产茸量关系的研究

Relationship Between Blood Protein Polymorphism and Antler Production in Gansu Wapiti

采用垂直板聚丙烯酰胺凝胶电泳技术,分析了66头甘肃马鹿的8个血液蛋白位点的基因频率、基因型频率和群体遗传变异指标,建立了产茸量与多态位点之间的线性相关模型。结果表明:甘肃马鹿各蛋白位点中,Tf、Hb、Alb、Pa、Prt1、Prt2、血清ES分别有3、1、1、2、2、2和3种基因型,未检测到红细胞ES;Tf和Prt2的基因型频率偏离Hardy-Wein-berg平衡;Tf的变异程度最大(杂合度为0.5863),杂合度的大小顺序为Tf>Prt2>ES>Pa>Prt1>Hb=Alb,群体的平均杂合度为0.3505;各多态位点中仅有Prt1与产茸量之间存在显著正相关(P<0.05),而且Prt1的AA型产茸量明显高于其他各位点基因型。由此可见,Prt1位点的AA型可用来标记甘肃马鹿的品种特征。

Eight blood protein loci（Tf, H b, Alb, Pa, Prtl, Prt2, serum ES and blood ES）of 66 deers in Gansu Wapiti were detected by using vertical polyamylamide gel electrophoresis. General linear model about blood protein loci and antler production were found. The results showed that the genotype of Tf, Hb, Alb, Pa,Prt1, Prt2 and serum ES was 3,1,1,2,2,2 and 3 respectively. Blood ES was not existent. It also showed that all loci of genotype lie in Hardy-Weinberg equilibrium state except Tf and Prt2 loci. The variability of Tf was the biggest（the heterozygosity was 0. 586 3）,the sequence of heterozygosity was Tf〉Prt2〉ES〉Pa〉Prt1〉Hb-Alb, and the average heterozygosity of polymorphism loci for Gansu Wapiti was 0. 350 5. The effect value analysis showed that it was up to significant differencc（P〈0.05） for AA type of Prtl locus and the antler production, therefore,the AA type in locus Prtl could be used as the deer＇s marker for the breeding of Gansu Wapiti.